Description
The ImmuChrom Alpha-1 Antitrypsin ELISA Kit is intended for the quantitative determination of alpha-1-antitrypsin (A1AT) in human stool or serum samples.
Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitor. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophiles. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes.
Type
Sandwich ELISA, HRP-labelled antibody
Other names
A1AT, α-1-AT, SERPINA1, SERPIN A1, serum trypsin inhititor, alpha 1 proteinase inhibitor, Alpha-1-antiproteinase, fecal Alpha-1-Antitrypsin, faecal Alpha-1-Antitrypsin
Principle of Method
The α-1-AT -ELISA test determines human alpha-1-antitrypsin according to the “sandwich”-principle. α-1-AT in sample, standard and controls binds to antibodies, which are coated to the microtiterplate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiterplate reader. The α-1-AT concentration can be calculated from the standard curve.
Sample Types
Stool, Serum
Sample Volume
Stool: 100 mg (collected sample)
Serum: 0.0004 µL (actual amount of sample loaded in well after dilution)
Standard Curve
Calibration Range: 3.3 ng/ml - 90 ng/ml
Limit of Detection
1.5 ng/ml
Intra-assay (Within-Run) C.V.
8.7%
Inter-assay (Run-to-Run) C.V.
11.9%
Resources
- Manual/PDS
- ARUP Laboratories validation poster: Performance Evaluation of a Polyclonal Based Fecal Alpha-1-Antitrypsin ELISA
- ARUP Laboratories validation study: Performance Evaluation of an ELISA for the Quantitative Measurement of α1-Antitrypsin in Stool
Ilex Life Sciences LLC is an official distributor of Immuchrom GmbH.