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Description
Lyophilized Atelocollagen (Cat. No. PRO-2001), is characterized by rich, highly purified Type I collagen content that retains its superior functionality and physical/mechanical properties. PRO-2001 is extracted from porcine tendon tissue sourced from strictly controlled herds, complying with all EU regulations. The proprietary pepsin enzyme-based extraction method used removes telopeptides at the non-helical N and C termini, resulting in a triple helix configuration that exhibits reduced immunogenicity.
Applications
Extracted atelocollagen is biocompatible and biodegradable and thus suitable for a wide range of research applications, such as:
- In vitro cell cultures
- Cell functional assays (invasion assay, proliferation assay, etc.)
- Soft 3D cell cultures (e.g. tumor assays)
Figure 1. Promed atelocollagen used as chemoattractant in cell invasion assay. Addition of atelocollagen to the substrate (A) promotes cell migration more efficiently than regular substrate (B) as quantified in graph (C).
Specifications
| Manufacturer | Promed Bioscience Ltd. |
| CAS Registry No. | 9007-34-5 |
|
Collagen Purity |
> 95% (by SDS/Western blotting) |
| Appearance | White/Off-white lyophilizate |
|
Source |
Porcine tendon |
|
Country of Animal Origin |
Cyprus |
| BSE Risk | None |
| Amino Acid Analysis | Typical/characteristic profile |
| Electrophoretic Pattern | Characteristic pattern, visible α, β, γ bands |
| Sterility/Microbiological testing | Pass (0 cfu/g) |
| Heavy Metals Analysis | < 1 ppm |
| Sterilization Method | Produced under strict aseptic conditions |
| Cell Attachment Assay | Pass |
| Cell Toxicity Assay | Pass |
Reconstitution
- PRO-2001 may be reconstituted to a concentration of 0.5 - 2.0 mg/ml when used as a coating agent and as chemoattractant.
- For the production of collagen gels, a concentration of 3.0 mg/ml may be used (slow gelation assays - cells will sink to the bottom of the gel).
- To reconstitute, add 0.1M acetic acid or 0.01M HCl to the vial and let it stand without stirring. Warming to 25˚C may be necessary for lyophilized atelocollagen to dissolve completely (clear solution).
Storage/Expiration
Product ships at ambient temperature. Upon receipt, store the product at the temperature recommended below:
- Long term: storage of lyophilized product at -20°C is recommended, with a storage life of 24 months from the date of manufacturing.
- Short term: storage of reconstituted product at 2-8°C is recommended, for up to 6 months from the date of reconstitution.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, cosmetic, supplement, or food additive.
Other Names
Type 1 Collagen, Type I Collagen, Collagen-1
Documents
Related Products
- Atelocollagen Ultrapure, Porcine Type I, Enzymatic Extraction, Dialyzed, Lyophilized
- Telocollagen, Porcine Type I, Acidic Extraction, Lyophilized
- Attachment Factors
-
Assays for Collagen Analysis
- 3-D Life Biomimetic Hydrogels
- Ossiform P3D Scaffolds: bone-like 3D cell culture systems
Ilex Life Sciences LLC is an authorized distributor of Promed Biosciences Ltd products.