The Biocolor Cell-Clock™ Cell Cycle Assay is a live cell detection and measurement system that can be employed to monitor the four major phases of the mammalian cell cycle during in vitro culture. The assay uses a redox dye that is imported by cycling cells.
Following dye uptake and incubation a distinct color change occurs within cells, with particular color changes being associated with cells in the G0-G1, S, G2 and M Phases.
The ratio of cells within the different phases can be calculated using this assay supplemented by visible evidence from photomicrographs. The calculation of phase ratios is obtained by computer software analysis (ImageJ) of the digitized images of photomicrographs.
CHO Cells stained with CellClock™ Dye Reagent
Live cell detection and measurement system
The image processing software – ImageJ is available for most operating systems, free of charge, from https://imagej.nih.gov/ij/. This software can be used to obtain ratios of cells within the different cell cycle phases. Full instructions are available in the product manual.
Cell Cycle Assay, Cell cycle assay kit, Cell Cycle phase determination assay
Ilex Life Sciences LLC is an official distributor of Biocolor Ltd.