Description
Ultra pure Actin, Alpha Cardiac Muscle 1, Native Protein is purified from bovine heart and has a molecular mass of 43,000 dalton.
Summary
Alpha-cardiac actin belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in the cardiac actin have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
Other Names
Actin alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC, UniProtKB - Q3ZC07 (ACTC_BOVIN)
Source
Bovine heart.
Formulation
Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1 mg/ml solution containing 10mM Tris-acetate buffer pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.5% SDS.
Purity
Greater than 98.0%.
Reconstitution
It is recommended to reconstitute the lyophilized Cardiac Actin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Cardiac-Actin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Cardiac Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Applications
- Protein standard in 1D and 2D SDS gelelectrophoresis
- Immunoassays
- Immunization
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
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