Description
NOTE: Higher-purity Type I Collagen is now available at a lower cost here: Promed Bioscience High-Purity Collagen
Mouse Collagen-I is a natural protein purified from Mouse tail tendon. Collagen-I is purified by proprietary chromatographic techniques.
Purified Type I Collagen is used in many research applications such as cell culture surface coatings, tissue engineering scaffolds, 3D bioprinting, and cell functional assays.
Summary
Collagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
Type I Collagen is by far the most abundant protein in all vertebrates. It assembles into fibers that form the structural and mechanical scaffold (matrix) of bone, skin, tendons, cornea, blood vessel walls and other connective tissues. Type I collagen consists of two α1(I) chains and one α2(I) chain.
Other Names
Murine Type 1 Collagen, Type I Collagen, Collagen-1
Source
Mouse tail tendon.
Formulation
Filtered white lyophilized (freeze-dried) powder. Collagen-I was lyophilized without additives.
Purity
Purity > 90.0%.
Reconstitution
We recommend to add 0.5 M acetic acid, pH 3 at 4°C to prepare a working stock solution not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Collagen-I, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-I should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting:
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications