Description
Bovine Collagen-III is a natural protein purified from washed dissected bovine tissue into dilute acetic acid. Collagen-III is purified by proprietary chromatographic techniques.
Summary
Collagen, a major component of the extracellular matrix (ECM), is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used ECM protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
Collagen-III, also known as type III collagen, is the second most abundant collagen in tissues; it is especially common in tissues with elastic properties such as skin, lungs, intestinal walls, and blood vessel walls. Collagen-III contains three alpha-1 chains and is structurally and functionally similar to other fibrillar collagens. Like collagen I, it is synthesized as procollagen; however, the mature fibrillar type III form retains the N-terminal propeptide.
Source
Bovine washed dissected tissue.
Formulation
Filtered white lyophilized (freeze-dried) powder. Collagen-III was lyophilized without additives.
Purity
Purity > 90.0%.
Reconstitution
We recommend to add 20 mM acetic acid to prepare a working stock solution of 1-5 mg/ml, which can then be further diluted to other aqueous solutions. Suspension should be shaken or stirred for several hours or overnight between 2-8°C.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Collagen-III, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Collagen-III should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
Type III Collagen, Type 3 Collagen, Collagen-3, COL3A1, Collagen Type III Alpha-1 Chain