Description
Bovine Collagen-IV is a natural protein purified from bovine placenta. Collagen-IV is purified by proprietary chromatographic techniques.
Collagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
Type IV collagen is a major component of the basement membrane, which is the specialized sheet-like ECM of multicellular tissues that exists around certain cell types (e.g., skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes).
Source
Bovine placenta.
Formulation
Filtered white lyophilized (freeze-dried) powder. Collagen-IV was lyophilized without additives.
Purity
Purity > 90.0%.
Reconstitution
We recommend to reconstitute the lyophilized Collagen-IV in 20 mM acetic acid not less than 1 mg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Collagen-IV, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-IV should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
Type IV Collagen, Type 4 Collagen, Collagen-4, COL4A1, Collagen alpha-1(IV) chain
RESOURCES
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting:
- UniProt Knowledgebase: UniProtKB - Q7SIB2 (CO4A1_BOVIN): Collagen alpha-1(IV) chain
- PubChem Gene Summary: COL4A1 - collagen type IV alpha 1 chain (cattle)
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications