Human Fibronectin purified from Human plasma. Molecular weight of 440 kDa.
Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50 µg/ml.
Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis, thrombosis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE.
Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane- bound Fibronectin receptors on cell surfaces.
The Human Fibronectin was lyophilized from a non sterile 2 mg/ml buffer of 10mM sodium phosphate, pH 7.5 and 0.15M NaCl.
We suggest reconstituting 1 mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2 mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea.
When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
At ambient temperature. Upon receipt, store the product at 4ºC
Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
This product is intended to Laboratory Research Use Only. This product may not be used a drug, agricultural product, food additives.
Cold insoluble globulin, FINC, LETS, MSF, FN
Want to learn more about Fibronectin? We have compiled the links below which contain information that you may find interesting:
- UniProt Knowledgebase: UniProtKB - P02751 (FINC_HUMAN): Fibronectin
- PubChem Gene Summary: FN1 - fibronectin 1 (human)
- To, Wing S, and Kim S Midwood. “Plasma and cellular fibronectin: distinct and independent functions during tissue repair.” Fibrogenesis & tissue repair vol. 4 21. 16 Sep. 2011, doi:10.1186/1755-1536-4-21
- Blog: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin