L-Asparaginase is an enzyme that depletes L-Asparagine, an important nutrient for cancer cells, resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease. The rationale behind asparaginase is that it takes advantage of the fact that acute lymphoblastic leukemia cells are unable to synthesize the non-essential amino acid asparagine whereas normal cells are able to make their own asparagine. These leukemic cells depend on circulating asparagine. Asparaginase catalyzes the conversion of L-asparagine to aspartic acid and ammonia. This deprives the leukemic cell of circulating asparagine.
L-Asparaginase was purified from E. coli ASI.357
CAS Registry No.
The L-Asparaginase enzyme was lyophilized with no additives.
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized L-Asparaginase in 18MOhm water at 1 mg/ml.
One unit of enzyme catalyzes hydrolyzation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C.
At ambient temperature. Upon receipt, store the product at -20ºC
Product stable though labelled expiration date when stored at –20°C or 2 weeks at 4°C.
This product is intended for Laboratory Research Use Only. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
L-ASNase, L-asparagine amidohydrolase, ansA