Introduction
Intrinsically developed for the cultivation of murine embryonic cells, DMEM (Dulbecco's Modified Eagle Medium) is tailor-made for the cultivation of a broad range of cells, especially if the medium is supplemented with FBS. DMEM is an Eagle medium modification with a fourfold content of amino acids and vitamins. DMEM with 1.0 g/L Glucose is the standard medium, whereas DMEM with 4.5 g/L Glucose is for cells which have a high energy demand.
Composition
PAN-Biotech DMEM, w: 1.0 g/L Glucose, w/o: L-Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3
Specifications
Cat. No. P04-01500
| Bicarbonate: | With 3.7 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | Low Glucose (1.0 g/L) |
| Glutamine: | No glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | DMEM |
| Size: | Various |
| Sodium Pyruvate: | With Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Documents
Product References
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Grubwieser, Philipp et al. “Quantification of Macrophage Cellular Ferrous Iron (Fe2+) Content Using a Highly Specific Fluorescent Probe in a Plate Reader.” Bio-protocol vol. 14,3 e4929. 5 Feb. 2024, doi:10.21769/BioProtoc.4929
- Odendaal, Christoff et al. “Personalised modelling of clinical heterogeneity between medium-chain acyl-CoA dehydrogenase patients.” BMC biology vol. 21,1 184. 4 Sep. 2023, doi:10.1186/s12915-023-01652-9
- Brigo, Natascha et al. “Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.” Cells vol. 12,8 1164. 14 Apr. 2023, doi:10.3390/cells12081164
- Brigo, Natascha et al. “Continuous Measurement of Reactive Oxygen Species Formation in Bacteria-infected Bone Marrow-derived Macrophages Using a Fluorescence Plate Reader.” Bio-protocol vol. 13,3 e4604. 5 Feb. 2023, doi:10.21769/BioProtoc.4604
- Nandan, Akhilesh et al. “Cells use molecular working memory to navigate in changing chemoattractant fields.” eLife vol. 11 e76825. 6 Jun. 2022, doi:10.7554/eLife.76825
- Brigo, Natascha et al. “Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein.” Bio-protocol vol. 12,11 e4440. 5 Jun. 2022, doi:10.21769/BioProtoc.4440
- Steuer, Anna et al. “Cell stimulation versus cell death induced by sequential treatments with pulsed electric fields and cold atmospheric pressure plasma.” PloS one vol. 13,10 e0204916. 12 Oct. 2018, doi:10.1371/journal.pone.0204916
- Stanoev, Angel et al. “Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network.” Cell systems vol. 7,3 (2018): 295-309.e11. doi:10.1016/j.cels.2018.06.006
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