Introduction
In normal whole-blood, erythrocyte concentration is a thousand-fold higher than leucocyte concentration, making erythrocytes an impediment to investigation or analysis of leucocytes. Erythrolyse buffers or Red-Cell-Lysing-Buffer is intended for the lysis of red blood cells in samples with white blood cells as for example EDTA-/citrate/heparin-treated whole-blood, Buffy Coats (PBMCs, leucocyte films), and bone marrow. Red-Blood-Cell-Lysis-Buffer is specially used for lysing human erythrocytes in single-cell suspensions of peripheral blood and mouse hematopoietic tissues such as spleen.
This buffer contains ammonium chloride, which lyses red cells, with minimal effect on lymphocytes when used as instructed. Nucleated red cells are not effectively lysed with ammonium chloride.
Application
Removal of red blood cells (RBCs) from samples is a necessary step prior to flow cytometric analysis, immunophenotyping, immunofluorescence staining, cell culture, cytospin, cytogenetics, and cell sorting of leukocytes.
Advantages
- Ready-to-use
- Easy application
- Minimal effect on lymphocytes
Specifications
Cat. No. P10-90100
| Bicarbonate: | with Potassium Bicarbonate |
|---|---|
| Ca/Mg | without Ca and Mg |
| Concentration | 1x |
| Endotoxin Content: | Endotoxin Content unspecified |
| Liquid / Powder: | liquid |
| Phenol Red: | without Phenol Red |
| Product Category: | Balanced Salt Solutions |
| Product Type: | Erythrocyte Lysis Buffer (RBC Lysis Buffer) |
| Size: | various |
| Sterile: | Yes |
| Storage Temperature: | +2°C - RT |
Composition
This buffer contains ammonium chloride (NH4Cl), potassium hydrogencarbonate (KHCO3), and Ethylenediaminetetraacetic acid (EDTA). Erythrocyte Lysis Buffer (1x) is sterile filtered (0.2 μm) and contains no fixative and no preservatives.
Documents
Product References
- Haque, Akm Ashiqul et al. “RNA ImmunoGenic Assay: A Method to Detect Immunogenicity of in vitro Transcribed mRNA in Human Whole Blood.” Bio-protocol vol. 10,24 e3850. 20 Dec. 2020, doi:10.21769/BioProtoc.3850
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