NOTE: There is a new and improved medium available for serum-free, defined hybridoma cultivation: PAN-Biotech HybridBoost: Defined Serum-Free Hybridoma Medium
Description
PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3 is a serum-free and defined culture medium for in vitro cultivation and expansion of hybridoma cell lines. High yield and final concentration of monoclonal antibodies (mAb) can be achieved. As this product is free of hydrolysates, and only contains traces of animal-derived components (<0.1% w/v) and proteins (<0.1% w/v), it is suitable for the production of mAb in higher scales.
| Cell Type: | Hybridoma |
|---|---|
| Culture Type: | Suspension Culture |
| Liquid / Powder: | liquid |
| Product Category: | Serum-Free Media |
| Size: |
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| Sterile: | Yes |
Composition
PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3: ISF-1 has been manufactured with stable Glutamine. It also contains a surfactant. Therefore no additional supplement for agitated suspension culture is needed.
Suitability
Serum-free, defined hybridoma culture for production of monoclonal antibodies (mAb).
ISF-1 works well for a variety of hybridoma systems, but will not grow cholesterol dependent cell lines without further supplementation (Addition of a lipoprotein preparation or other source of cholesterol is needed for these cell lines). ISF-1 is compatible with most antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin or Fungizone. The use of antibiotics could reduce the yield and productivity of hybridoma cell lines.
This product has been tested in different cell culture system, including stationary culture, spinner, roller bottles and bioreactors.
Figure 1.: Influence of different media on cell growth, viability and productivity. Hybridoma cells were cultivated in T-flasks. After adaption of the cells to the different media, cell growth, viability and productivity were measured after one week of cultivation. Productivity is shown in comparison to 10 % FBS-containing medium.
Storage conditions & stability
Storage: at +2°C - +8°C in the dark.
Stability: 1 year from date of production
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