PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3, 1000 ml (catalog no. P04-995968)

PAN-Biotech ISF-1 Serum-free Hybridoma Medium

P04-995968
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$95.00
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$95.00
Regular price
Available January 20th
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Description

PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3 is a serum-free and defined culture medium for in vitro cultivation and expansion of hybridoma cell lines. High yield and final concentration of monoclonal antibodies (mAb) can be achieved. As this product is free of hydrolysates, and only contains traces of animal-derived components (<0.1% w/v) and proteins (<0.1% w/v), it is suitable for the production of mAb in higher scales.

    Cell Type: Hybridoma
    Culture Type: Suspension Culture
    Liquid / Powder: liquid
    Product Category: Serum-Free Media
    Size: 1000 ml
    Sterile: Yes

    Composition

    PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3: ISF-1 has been manufactured with stable Glutamine. It also contains a surfactant. Therefore no additional supplement for agitated suspension culture is needed.

    Suitability

    ISF-1 works well for a variety of hybridoma systems, but will not grow cholesterol dependent cell lines without further supplementation (Addition of a lipoprotein preparation or other source of cholesterol is needed for these cell lines). ISF-1 is compatible with most antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin or Fungizone. The use of antibiotics could reduce the yield and productivity of hybridoma cell lines.

    This product has been tested in different cell culture system, including stationary culture, spinner, roller bottles and bioreactors.

      Storage conditions & stability

      Storage: at +2°C - +8°C in the dark.

      Stability: 1 year from date of production

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