PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3 is a serum-free and defined culture medium for in vitro cultivation and expansion of hybridoma cell lines. High yield and final concentration of monoclonal antibodies (mAb) can be achieved. As this product is free of hydrolysates, and only contains traces of animal-derived components (<0.1% w/v) and proteins (<0.1% w/v), it is suitable for the production of mAb in higher scales.
|Culture Type:||Suspension Culture|
|Liquid / Powder:||liquid|
|Product Category:||Serum-Free Media|
PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3: ISF-1 has been manufactured with stable Glutamine. It also contains a surfactant. Therefore no additional supplement for agitated suspension culture is needed.
ISF-1 works well for a variety of hybridoma systems, but will not grow cholesterol dependent cell lines without further supplementation (Addition of a lipoprotein preparation or other source of cholesterol is needed for these cell lines). ISF-1 is compatible with most antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin or Fungizone. The use of antibiotics could reduce the yield and productivity of hybridoma cell lines.
This product has been tested in different cell culture system, including stationary culture, spinner, roller bottles and bioreactors.
Storage conditions & stability
Storage: at +2°C - +8°C in the dark.
Stability: 1 year from date of production
Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.