Introduction
MCDB 131 is a medium that was originally developed by Knedler and Ham for the cultivation of human microvascular endothelial cells (HMVEC) under reduced serum content. For this purpose it is often supplemented with dialyzed serum, EGF, and hydrocortisone. MCDB 131 Medium is also suitable for other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. Additionally, MCDB 131 has been used for definitive endoderm differentiation of human pluripotent stem cells (hPSCs) as well as a basal medium for mesenchymal stem cells (MSCs) differentiation.
MCDB 131 Medium is formulated with several components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. This formulation allows the medium to be supplemented with very low levels of serum or defined components.
Composition
PAN-Biotech MCDB 131, w/o: L-Glutamine, w: 1.176 g/L NaHCO3
Specifications
Cat. No. P04-80057
| Bicarbonate: | With 1.176 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | No Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MCDB 131 |
| Size: | Various |
| Sodium Pyruvate: | With Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Documents
Product References
- Jalil, Sami et al. “Genetic and functional correction of argininosuccinate lyase deficiency using CRISPR adenine base editors.” American journal of human genetics vol. 111,4 (2024): 714-728. doi:10.1016/j.ajhg.2024.03.004
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