Introduction
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble the protein composition of mammalian cells. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for many further modifications.
Composition
PAN-Biotech MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO3
Specifications
Cat. No. P04-08500: Contains Earle’s Balanced Salt Solution (EBSS)
| Bicarbonate: | With 2.2 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | L-Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MEM |
| Size: | Various |
| Sodium Pyruvate: | Without Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
Documents
Product References
- Adihou, Hélène et al. “A protein tertiary structure mimetic modulator of the Hippo signalling pathway.” Nature communications vol. 11,1 5425. 27 Oct. 2020, doi:10.1038/s41467-020-19224-8
- Schöler, Lara et al. “Establishment and clinical validation of an in-cell-ELISA-based assay for the rapid quantification of Rabies lyssavirus neutralizing antibodies.” PLoS neglected tropical diseases vol. 16,5 e0010425. 10 May. 2022, doi:10.1371/journal.pntd.0010425
- Paulus, Jannik, and Norbert Sewald. “Synthesis and Evaluation of a Non-Peptide Small-Molecule Drug Conjugate Targeting Integrin αVβ3.” Frontiers in chemistry vol. 10 869639. 11 Apr. 2022, doi:10.3389/fchem.2022.869639
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