Introduction
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble the protein composition of mammalian cells. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for many further modifications.
A GMP Grade version is now available: PAN-Biotech MEM Eagle w: EBSS, w/o: L-Glutamine, w: 2.2 g/L NaHCO3, GMP Grade.
Composition
PAN-Biotech MEM Eagle w: EBSS, w/o: L-Glutamine, w: 2.2 g/L NaHCO3
Specifications
Cat. No. P04-08050: Contains Earle’s Balanced Salt Solution (EBSS)
| Quality: | Research Use Only (RUO) |
|---|---|
| Bicarbonate: | With 2.2 g/L Sodium Bicarbonate |
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | No Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MEM |
| Size: | 500 ml bottles |
| Sodium Pyruvate: | Without Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Documents
Product References
- Caetano-Pinto, Pedro et al. “In Vitro Characterization of Renal Drug Transporter Activity in Kidney Cancer.” International journal of molecular sciences vol. 23,17 10177. 5 Sep. 2022, doi:10.3390/ijms231710177
- Arhzaouy, Khalid et al. “VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle.” Autophagy vol. 15,6 (2019): 1082-1099. doi:10.1080/15548627.2019.1569933
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