Introduction
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble the protein composition of mammalian cells. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for many further modifications.
MEM-NEAA: The addition of non-essential amino acids (NEAA) to MEM (MEM-NEAA) has increased the suitability of this medium for a broad range of applications.
Specifications
Cat. No. P04-08509: Contains Earle’s Balanced Salt Solution (EBSS) and non-essential amino acids (NEAA).
| Bicarbonate: | With 2.2 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | No Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MEM |
| Size: | Various |
| Sodium Pyruvate: | Without Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer | PAN-Biotech GmbH |
| Country of Origin | Germany |
Documents
Product References
- Schmidt, Sara C et al. “Microscopic clusters feature the composition of biochemical tetraspanin-assemblies and constitute building-blocks of tetraspanin enriched domains.” Scientific reports vol. 14,1 2093. 24 Jan. 2024, doi:10.1038/s41598-024-52615-1
- Reppert, Nikolas, and Thorsten Lang. “A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn.” Scientific reports vol. 12,1 4494. 16 Mar. 2022, doi:10.1038/s41598-022-07243-y
- Mertins, Jasmin et al. “The mesoscale organization of syntaxin 1A and SNAP25 is determined by SNARE-SNARE interactions.” eLife vol. 10 e69236. 15 Nov. 2021, doi:10.7554/eLife.69236
- Finke, Jérôme et al. “HPV caught in the tetraspanin web?.” Medical microbiology and immunology vol. 209,4 (2020): 447-459. doi:10.1007/s00430-020-00683-1
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