Introduction
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble the protein composition of mammalian cells. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for many further modifications.
Composition
PAN-Biotech MEM Eagle w: EBSS, w: stable Glutamine, w: 2.2 g/L NaHCO3
Specifications
Cat. No. P04-09500: Contains Earle’s Balanced Salt Solution (EBSS)
| Bicarbonate: | With 2.2 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | With Stable Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MEM |
| Size: | Various |
| Sodium Pyruvate: | Without Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Documents
Product References
- Kashyap, Purba et al. “An optogenetic method for the controlled release of single molecules.” Nature methods vol. 21,4 (2024): 666-672. doi:10.1038/s41592-024-02204-x
- Peradinovic, Josip et al. “Ageing-Induced Decline in Primary Myeloid Cell Phagocytosis Is Unaffected by Optineurin Insufficiency.” Biology vol. 12,2 240. 3 Feb. 2023, doi:10.3390/biology12020240
- Sotolongo Bellón, Junel et al. “Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane.” Cell reports methods vol. 2,2 100165. 4 Feb. 2022, doi:10.1016/j.crmeth.2022.100165
- Heuberger, Julian et al. “Epithelial response to IFN-γ promotes SARS-CoV-2 infection.” EMBO molecular medicine vol. 13,4 (2021): e13191. doi:10.15252/emmm.202013191
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