Pancell T: Serum-Free, Chemically-Defined T-Cell Expansion Medium

Description

PAN-Biotech Pancell T is a chemically defined and protein-free (< 0.02%) medium for serum-free cultivation and proliferation of lymphocytes from full blood. The medium can be used for the enrichment and expansion of T-cells in vitro.

Pancell T enables:

• Enrichment of T-cells from PBMC / full blood, after stimulation (e.g., with PHA-L or anti-CD3)
• Stable performance > 4 passages
• Long-term cultivation of immortalized T-cells
• CD3+ > 80%

Figure 1: : PBMC cultivated in Pancell T, five days after stimulation.

Composition

Pancell T: T-Cell Expansion Medium (Cat. No. P04-710414X10, P04-710414X20): consists of a balanced mixture of salts, amino acids, vitamins, trace elements and hormones for optimized growth.

Pancell T: T-Cell Expansion Kit (Cat. No. P04-710414KX20): in addition to Pancell T medium, this kit includes PHA-L as supplement for the stimulation of lymphocytes (Cat. No. P05-01010M) and a cytokines supplement (Cat. No. P04-413S).

Storage conditions & stability

Pancell T: T-Cell Expansion Medium: Store at +2°C - +8°C in the dark.

Pancell T supplemented with growth factors can be safely stored up to 3 months at 2-8°C in the dark. Repeated warming / cooling cycles and exposure to light should be avoided.

PHA-L (lyophilized): Store at +2°C - +8°C in the dark.

Pancell Growth Supplement: Store at -20°C.

Documents

• Product Datasheet (PDS)

Related Products

• Pancoll Human: Cell Separating Solution, Density 1.077 g/ml

Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.