QuickZyme Human MMP-2 Activity Assay principle
QuickZyme Human MMP-2 Activity Assay
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QuickZyme Human MMP-2 Activity Assay

QZBMMP2HV2
Regular price
$940.00
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$940.00
Regular price
$1,050.00
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Description

The QuickZyme Human MMP-2 Activity Assay enables you to specifically measure in biological samples both active MMP-2, as well as (pro)MMP-2, which is activated on the plate by APMA. It can be used for the measurement of MMP-2 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.

This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.

Features

  • Measures endogenous active MMP-2 (naturally occurring) or total active MMP-2 (following activation with APMA).

  • Samples: cell culture conditioned medium, serum, plasma, urine and tissue homogenates.

  • Quantitative.

  • Range: 0.02 – 16 ng/ml.

  • Sensitivity: 0.04 ng/ml for 2 h incubation with detection reagent; 0.02 ng/ml for 6 h incubation with detection reagent; 4 pg/ml for overnight incubation

  • Ease-of-use: Equivalent to ELISA.

    Type

    Enzyme activity immunoassay

    Human MMP-2 Activity Assay principle

    Applications

    • cell culture conditioned medium, serum, plasma, urine and tissue homogenates

    Sensitivity

    • 0.04 ng/ml (2 hr incubation)
    • 0.02 ng/ml (6 hr incubation)
    • 4 pg/ml (o/n incubation)

    Quantitative Measurement

    Typical data at 4 hours incubation: QuickZyme Human MMP-2 Activity Assay

    Typical data with 4 hours incubation.

    Storage

    Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.

    Opened kit / reconstituted reagents: Please refer to kit manual.

    Other names

    Matrix metalloproteinase-2, Gelatinase A, TBE-1, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, MMP-2, TBE-1, PEX, MMP2 CLG4A

    Resources

    Manual/PDS

    Product Brochure 

    Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.