Description
Quantify both endogenous active MMP-2 and total MMP-2
The QuickZyme Human MMP-2 Activity Assay enables you to specifically measure in biological samples both active MMP-2, as well as (pro)MMP-2, which is activated on the plate by APMA. It can be used for the measurement of MMP-2 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.
This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.
Features
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Measures both endogenous active MMP-2 (naturally occurring) and total active MMP-2 (following activation with APMA).
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Samples: cell culture conditioned medium, serum, plasma, urine and tissue homogenates.
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Quantitative.
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Range: 0.02 – 16 ng/ml.
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Sensitivity: 0.04 ng/ml for 2 h incubation with detection reagent; 0.02 ng/ml for 6 h incubation with detection reagent; 4 pg/ml for overnight incubation
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Ease-of-use: Equivalent to ELISA.
Type
Enzyme activity immunoassay
Applications
- cell culture conditioned medium, serum, plasma, urine and tissue homogenates
Sensitivity
- 0.04 ng/ml (2 hr incubation)
- 0.02 ng/ml (6 hr incubation)
- 4 pg/ml (o/n incubation)
Quantitative Measurement
Typical data with 4 hours incubation.
Storage
Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.
Opened kit / reconstituted reagents: Please refer to kit manual.
Other names
Matrix metalloproteinase-2, Gelatinase A, TBE-1, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, MMP-2, TBE-1, PEX, MMP2 CLG4A
Resources
Related Products
Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.