QuickZyme Human MMP-8 Activity Assay principle
QuickZyme logo
  • Load image into Gallery viewer, QuickZyme Human MMP-8 Activity Assay principle
  • Load image into Gallery viewer, QuickZyme logo

QuickZyme Human MMP-8 Activity Assay

QZBMMP8H
Regular price
$890.00
Sale price
$890.00
Regular price
$994.00
Temporarily out of stock
Unit price
per 
Shipping calculated at checkout.

Description

The QuickZyme Human MMP-8 Activity Assay enables you to specifically measure in biological samples both active human MMP-8, as well as (pro)MMP-8 which is activated on the plate by APMA. It can be used for the measurement of MMP-8 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.

This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.

Features

  • Measures endogenous active MMP-8 (naturally occurring) or total active MMP-8 (following activation with APMA).
  • Samples: cell culture conditioned medium, serum, plasma, urine, saliva and tissue homogenates.
  • Quantitative.
  • Range: 10 – 24000 pg/ml.
  • Sensitivity: 100 pg/ml for 2 h incubation with detection reagent; 24 pg/ml for 6 h incubation with detection reagent; 4 pg/ml for 24 h incubation with detection reagent.
  • Ease-of-use: Equivalent to ELISA.
  • Marker for neutrophils (PMN)

      Type

      Enzyme activity immunoassay

      QuickZyme Human MMP-8 Activity Assay principle

      Applications

      • cell culture conditioned medium, serum, plasma, urine, saliva, tissue homogenates

      Sensitivity

      • 100 pg/ml (2 hr incubation)
      • 24 pg/ml (6 hr incubation)
      • 4 pg/ml (24 hr incubation)

          Quantitative Measurement

          Typical data with 2 hours incubation: QuickZyme Human MMP-8 Activity Assay

          Typical data with 2 hours incubation. The shown data curve is provided for demonstration only.

          Storage

          Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.

          Opened kit / reconstituted reagents: Please refer to kit manual.

          Other names

          Matrix metalloproteinase-8, MMP8, Neutrophil collagenase, PMNL collagenase, PMNL-CL

          Resources

          Manual/PDS

          Product Brochure 

          Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.