QuickZyme Human MMP-9 Activity Assay principle
QuickZyme Logo
  • Load image into Gallery viewer, QuickZyme Human MMP-9 Activity Assay principle
  • Load image into Gallery viewer, QuickZyme Logo

QuickZyme Human MMP-9 Activity Assay

QZBMMP9H
Regular price
$890.00
Sale price
$890.00
Regular price
$994.00
Temporarily out of stock
Unit price
per 
Shipping calculated at checkout.

Description

The QuickZyme Human MMP-9 Activity Assay enables you to specifically measure in biological samples both active human MMP-9, as well as (pro)MMP-9 which is activated on the plate by APMA. It can be used for the measurement of MMP-9 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.

This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.

Features

  • Measures endogenous active MMP-9 (naturally occurring) or total active MMP-9 (following activation with APMA).
  • Samples: conditioned cell culture medium, serum, plasma, urine, synovial fluid and tissue homogenates.
  • Quantitative.
  • Range: 0.5 to 16 ng/ml (1 h incubation with detection reagent), 0.01-1 ng/ml (4 h incubation with detection reagent).
  • Sensitivity: 0.005 ng/ml.
  • Ease-of-use: Equivalent to ELISA.

        Type

        Enzyme activity immunoassay

        QuickZyme Human MMP-9 Activity Assay principle

        Applications

        • conditioned cell culture medium, serum, plasma, urine, synovial fluid, tissue homogenates

        Sensitivity

        • 0.1 ng/ml (1 hr incubation)
        • 0.005 ng/ml (4 hr incubation)

            Quantitative Measurement

            Typical data with 4 hours incubation: QuickZyme Human MMP-9 Activity Assay

            Typical data with 4 hours incubation. The shown data curve is provided for demonstration only.

            Storage

            Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.

            Opened kit / reconstituted reagents: Please refer to kit manual.

            Other names

            Matrix metalloproteinase-9, MMP9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

            Resources

            Manual/PDS

            Product Brochure 

            Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.