Description
Now hydroxyproline can be easily determined in small tissue samples with low collagen levels!
Dysregulation in collagen metabolism may result in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.
Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where it leads to increased triple helix stability. Hydroxyproline is formed post-translationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.
The QuickZyme Sensitive Tissue Hydroxyproline assay is a modification of the method described by Prockop and Udenfriend (Anal. Biochem.,1960, 1: 228-239). The assay measures the total amount of hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all the types of collagen present in the sample without discriminating between the types of collagen and between procollagen, mature collagen and collagen degradation products.
The QuickZyme Sensitive Tissue Hydroxyproline assay kit is the only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate enabling measurement in small samples with low hydroxyproline concentrations. In addition, no drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay.
Features
- Quantitative measurement of hydroxyproline
- Optimized for tissues with low collagen or hydroxyproline content ( such as liver) due to absence of matrix effects
- Samples: tissue homogenates, tissues
- Range: 6 to 300 μM. Sensitivity: 2.4 µM (0.3 µg/ml)
- Uses hydroxyproline as standard
- Ease-of-use: Equivalent to ELISA; Colorimetric read out
- Storage at room temperature
- This kit does not contain tubes and a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay
Type
Colormetric Assay
Applications
- Tissue, Tissue homogenates
Sensitivity
2.4 µM (0.3 µg/ml)
Quantitative Measurement
Negligible Matrix Effect
Many assays need (>5 fold ) dilution due to matrix effects. This new assay has matrix effect eliminated, making it possible to use non-diluted samples.
Storage
Unopened kit: Store at room temperature in the dark. Do not use kit components past the kit expiration date.
Opened kit / reconstituted reagents: The opened standard, assay buffer and enhancer solution should be stored light protected at 4°C. The other opened reagents should be stored light protected at RT and are stable for at least 1 month. The reconstituted detection reagent (A+B) should be used on the day of reconstitution.
Other Names/Synonyms
hydroxyproline residues
Documents
- Product Datasheet (PDS)
- Application note 1: How to choose your collagen assay?
- Application note 2: How to measure collagen in mouse tissues?
- Application note 3: Collagen per protein analysis in fresh, frozen and formalin-fixed tissues
Product References
- Gart, Eveline et al. “Translational characterization of the temporal dynamics of metabolic dysfunctions in liver, adipose tissue and the gut during diet-induced NASH development in Ldlr-/-.Leiden mice.” Heliyon vol. 9,3 e13985. 24 Feb. 2023, doi:10.1016/j.heliyon.2023.e13985
- Upchurch, Clint M et al. “Targeting oxidized phospholipids by AAV-based gene therapy in mice with established hepatic steatosis prevents progression to fibrosis.” Science advances vol. 8,28 (2022): eabn0050. doi:10.1126/sciadv.abn0050
- Seidel, Florine et al. “Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice.” International journal of molecular sciences vol. 23,18 10736. 14 Sep. 2022, doi:10.3390/ijms231810736
- Gart, Eveline et al. “Butyrate Protects against Diet-Induced NASH and Liver Fibrosis and Suppresses Specific Non-Canonical TGF-β Signaling Pathways in Human Hepatic Stellate Cells.” Biomedicines vol. 9,12 1954. 20 Dec. 2021, doi:10.3390/biomedicines9121954
- Sakai, Takahiro et al. “Effects of the Cytoplasm and Mitochondrial Specific Hydroxyl Radical Scavengers TA293 and mitoTA293 in Bleomycin-Induced Pulmonary Fibrosis Model Mice.” Antioxidants (Basel, Switzerland) vol. 10,9 1398. 31 Aug. 2021, doi:10.3390/antiox10091398
- van den Hoek, Anita M et al. “Beneficial effects of elafibranor on NASH in E3L.CETP mice and differences between mice and men.” Scientific reports vol. 11,1 5050. 3 Mar. 2021, doi:10.1038/s41598-021-83974-8
- van den Hoek, Anita M et al. “A Translational Mouse Model for NASH with Advanced Fibrosis and Atherosclerosis Expressing Key Pathways of Human Pathology.” Cells vol. 9,9 2014. 1 Sep. 2020, doi:10.3390/cells9092014
- Mayeux, Jessica M et al. “Development of experimental silicosis in inbred and outbred mice depends on instillation volume.” Scientific reports vol. 9,1 14190. 2 Oct. 2019, doi:10.1038/s41598-019-50725-9
- Ulmasov, Barbara et al. “An Inhibitor of Arginine-Glycine-Aspartate-Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis.” Hepatology communications vol. 3,2 246-261. 27 Dec. 2018, doi:10.1002/hep4.1298
Related Products
Further Reading
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting:
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications
Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.