Now hydroxyproline can be easily determined in small tissue samples with low collagen levels!
Dysregulation in collagen metabolism may result in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.
Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where it leads to increased triple helix stability. Hydroxyproline is formed post-translationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.
The QuickZyme Sensitive Tissue Hydroxyproline assay is a modification of the method described by Prockop and Udenfriend (Anal. Biochem.,1960, 1: 228-239). The assay measures the total amount of hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all the types of collagen present in the sample without discriminating between the types of collagen and between procollagen, mature collagen and collagen degradation products.
The QuickZyme Sensitive Tissue Hydroxyproline assay kit is the only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate enabling measurement in small samples with low hydroxyproline concentrations. In addition, no drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay.
- Quantitative measurement of hydroxyproline
- Optimized for tissues with low collagen or hydroxyproline content ( such as liver) due to absence of matrix effects
- Samples: tissue homogenates, tissues
- Range: 6 to 300 μM. Sensitivity: 2.4 µM (0.3 µg/ml)
- Uses hydroxyproline as standard
- Ease-of-use: Equivalent to ELISA; Colorimetric read out
- Storage at room temperature
- This kit does not contain tubes and a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay
- Tissue, Tissue homogenates
2.4 µM (0.3 µg/ml)
Negligible Matrix Effect
Many assays need (>5 fold ) dilution due to matrix effects. This new assay has matrix effect eliminated, making it possible to use non-diluted samples.
Unopened kit: Store at room temperature in the dark. Do not use kit components past the kit expiration date.
Opened kit / reconstituted reagents: The opened standard, assay buffer and enhancer solution should be stored light protected at 4°C. The other opened reagents should be stored light protected at RT and are stable for at least 1 month. The reconstituted detection reagent (A+B) should be used on the day of reconstitution.
- Application note 1: How to choose your collagen assay?
- Application note 2: How to measure collagen in mouse tissues?
- Application note 3: Collagen per protein analysis in fresh, frozen and formalin-fixed tissues
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting:
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications
Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.