QuickZyme Soluble Collagen Assay principle
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QuickZyme Soluble Collagen Assay (96-well plate format)

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The QuickZyme Soluble Collagen Assay kit is a quantitative, dye-binding method for the measurement of all types of fibrillar collagen, in a fast and simple 96-well plate format.


Colormetric Assay (Sirius Red Collagen Assay)

The QuickZyme Soluble Collagen Assay is based on binding of Sirius Red dye to collagen. Following binding of the dye, the collagen-dye complex precipitates, resulting in a colored pellet. This color can be released in an alkaline solution and then read with a microplate reader.

QuickZyme Soluble Collagen Assay principle


Type of Collagen Measured Measures all types of fibrillar collagen, species independent. Measures soluble or solubilized collagens.
Assay Range 0.5 - 10 μg/ml 
Sensitivity 0.69 ng/ml
Format 96-well plate
Sample Volume 0 -140 μl

Sample Types

  • conditioned cell culture media
  • cellular extracts
  • solubilized collagen fractions

Assay Run-Time

< 3 hours


      Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore quantitative measurement of collagen production is important in many disease related studies.

      The QuickZyme Soluble Collagen Assay measures various types of fibrillar collagen. The most abundant collagen types are collagen type I (skin, bone, vessels, connective tissue), type II (cartilage) and type III (skin, bone, connective tissue).

      This assay is specific for collagen. After heating at 50°C the signal is lost, since the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin. The assay is intended for collagen analysis in conditioned cell culture media and cellular extracts. The assay is not affected by serum up to at least 10%.

      QuickZyme Soluble Collagen Assay: Typical collagen standard curve in the range of 0.5 – 10 μg collagen/well (3.5 - 70 μg/ml; blank value 0.070)

      Typical collagen standard curve in the range of 0.5 – 10 μg collagen/well (3.5 - 70 μg/ml; blank value 0.070).

      Tutorial Video


      Unopened kit:

      • Store at 4°C. Do not use kit components past kit expiration date.

      Opened kit / reconstituted reagents:

      • Collagen standard dissolved in 20 mM HAc: store at 4°C up to 1 month
      • Dye solution: store at 4°C up to 1 month
      • Dilution buffer: store at -20°C up to 6 months
      • Wash buffer: store at -20°C up to 6 months
      • Detection solution: store either at 4°C or room temperature up to 1 month


      Product References

      • Kondo, Masahiro et al. “Dersimelagon, a novel oral melanocortin 1 receptor agonist, demonstrates disease-modifying effects in preclinical models of systemic sclerosis.” Arthritis research & therapy vol. 24,1 210. 1 Sep. 2022, doi:10.1186/s13075-022-02899-3
      • Kiseleva, Ekaterina et al. “Interaction between mesenchymal stem cells and myoblasts in the context of facioscapulohumeral muscular dystrophy contributes to the disease phenotype.” Journal of cellular physiology vol. 237,8 (2022): 3328-3337. doi:10.1002/jcp.30789
      • Quesnel, Katherine et al. “CCN1 expression by fibroblasts is required for bleomycin-induced skin fibrosis.” Matrix biology plus vol. 3 100009. 6 Jul. 2019, doi:10.1016/j.mbplus.2019.100009
      • Kano, Miyu et al. “Attenuation of murine sclerodermatous models by the selective S1P1 receptor modulator cenerimod.” Scientific reports vol. 9,1 658. 24 Jan. 2019, doi:10.1038/s41598-018-37074-9
      • Matsushita, Takashi et al. “BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance.” Science advances vol. 4,7 eaas9944. 11 Jul. 2018, doi:10.1126/sciadv.aas9944
      • Han, Zonglin et al. “Inhibition of prostaglandin E2 protects abdominal aortic aneurysm from expansion through regulating miR-29b-mediated fibrotic ECM expression.” Experimental and therapeutic medicine vol. 16,1 (2018): 155-160. doi:10.3892/etm.2018.6160
      • Goldstein, Benjamin D et al. “Chronic asthma and Mesenchymal stem cells: Hyaluronan and airway remodeling.” Journal of inflammation (London, England) vol. 14 18. 30 Aug. 2017, doi:10.1186/s12950-017-0165-4

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      Further Reading

      Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting:

       Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.