Description
Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein is a secreted protein (amino acids Glu33-Arg254). M-CSF is a disulfide-linked homodimer containing 2 x 222 amino acid chains.
Summary
Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - Q8JZQ0 (CSF1_RAT)].
Other Names
Bioactive Rat M-CSF, CSF-1, CSF1 gene product, MCSF
Source
HEK293 cells
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The recombinant M-CSF was lyophilized after extensive dialysis against PBS.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The ED50 of 3-4 ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
Reconstitution
It is recommended to reconstitute the M-CSF in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
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