Description
Recombinant Human Albumin (rHA), GMP Grade, expressed in Pichia pastoris, is a 66 kDa synthetic alternative to human serum albumin (HSA). HSA is a major protein found in human plasma that is responsible for various physiological functions. The rHA is a highly purified product that is free of animal or human components. It is developed as an alternative to HSA, to which it is structurally equivalent. The rHA offers a reliable and sustainable alternative to traditional HSA sources.
Features
- Available as a liquid solution or as a lyophilized powder.
- Expressed in yeast P. pastoris by recombinant DNA technology.
- Manufactured by yeast fermentation process and purified using chromatography methods.
- Purity levels ≥ 99% confirmed by orthogonal quality control tests.
- Antibiotic-free and solvent-free manufacturing process.
- Raw materials used are animal component free, TSE/BSE-free certified, and comply with IP/USP/EP.
- Manufactured in a GMP certified facility with ISO classified clean rooms, following ICH Q7 principles to meet international standards.
- Quality control testing as per ICH Q6 and Q1 guidelines.
- Product is packed in single-use sterile endotoxin-free containers.
Applications
Due to free hydrophobic regions albumins are used to solubilize lipids and stabilize other proteins such as e.g. growth factors in tissue culture applications. They are also used as blocking agents in Western blots or ELISA applications and as a protein standard. Albumin has a low molecular weight, is easily soluble in water and contains an excess of acidic amino acids.
- Cell and tissue culture media supplement
- Used as serum substitute in serum-free, xeno-free cell culture
- Medium component for cryopreservation and stem cell culture
- Stabilizer protein for pharmaceutical and biotech products
- Reduces unspecific binding in molecular biology assays
Specifications
| Product Category |
Media supplements |
| Product Type |
HSA / BSA |
|
CAS Registry No. |
70024-90-7 |
| Source |
Expressed in Pichia pastoris yeast |
| Liquid / Powder |
Liquid solution |
| Appearance |
Clear, slightly viscous and colorless to yellow amber solution. |
| Identification |
Peptide mapping |
| pH |
6.4 to 7.4 |
| Assay Total Protein by HPLC-GPC | 19.0% - 21.0% |
| Albumin Concentration | > 60 mg/ml |
| Albumin Content Purity by HPLC-GPC |
≥ 99.0% |
| Impurities: High molecular weight proteins by HPLC method |
≤ 1.0% |
| Host Cell Proteins |
≤ 100 ppm |
| Host Cell DNA |
≤ 10 ppm |
| Bacterial Endotoxin |
< 0.5 USP Endotoxin Unit/ml |
| Sterile | Yes |
| Storage Temperature | 2-8°C |
| Manufacturer |
Provis Biolabs pvt Ltd |
| Country of Origin |
India |
Usage
This product is intended for laboratory research and biomanufacturing use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Background
Albumins are monomeric proteins found in body fluids and tissues. Human serum albumin (HSA) is the most abundant protein in human blood plasma with a molecular weight of about 66.5 kDa. The major biological function of albumin is to regulate the colloidal osmotic blood pressure. In addition, albumin acts as carrier protein for hormones, fatty acids and other compounds, and buffers pH. The reference range for albumin concentration in serum is approximately 35-50 g/L.
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Ilex Life Sciences LLC is an authorized distributor of Provis Biolabs.