Description
Recombinant Trypsin, GMP Grade, expressed in Pichia pastoris, is an enzyme with 223 amino acids and molecular weight of 23.5 kDa. Trypsin is a serine protease that cleaves peptide bonds on the carboxyl side of lysine and arginine residues, playing a crucial role in protein digestion and cell culture applications. Recombinant Trypsin offers improved purity, stability, and control compared to traditional animal-derived trypsin.
Features
- Available as a lyophilized power or liquid solution.
- Expressed in yeast P. pastoris by recombinant DNA technology.
- High specific activity ≥180 U/mg.
- Purity ≥ 70% (β-Trypsin)
- Optimal pH range from 7.5 to 8.5 for efficient enzymatic activity.
- Antibiotic-free manufacturing process.
- Raw materials used are animal component free, TSE/BSE certified, and comply with IP/USP/EP.
- Manufactured in a GMP certified facility with ISO classified clean rooms, following ICH Q7 principles to meet international standards.
Applications
- Adherent cell dissociation and passaging in cell culture.
- Protein digestion for mass spectrometry analysis.
- Recombinant protein production and purification.
- Tissue engineering and regenerative medicine.
- Production of insulin.
Specifications
| Product Category |
Cell Dissociation Enzymes |
|
Product Type |
Trypsin |
| CAS Registry No. |
9002-07-7 |
| Source |
Expressed in Pichia pastoris yeast |
|
Liquid/Powder |
Lyophilized powder |
| Appearance |
White to off-white, odorless powder |
| Solubility (10 mg/ml) |
Clear colorless solution |
| Size | Various, bulk sizes on request |
| Enzyme Activity by USP Method |
Not less than 2500 USP Units/mg |
| Protein Content by UV 280 Method |
95.0% ± 10.0% |
| Specific Activity |
Not less than 3800 USP Units/mg (BAEE) |
| Purity by RP-HPLC |
|
| Yeast Host Cell Protein Residue |
≤ 0.01% |
| Yeast Host Cell DNA Residue |
≤ 10.0000 ng/mg |
| Endotoxin Content (LAL Test) |
≤ 20.0 EU/mg |
| Total Microbial Count (TVAC/TYMC) |
|
| Pathogens | Free of E. coli, Salmonella sp., S. aureus, P. aeruginosa. |
| Storage Temperature | 2-8°C |
| Manufacturer | Provis Biolabs pvt Ltd |
| Country of Origin | India |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.
Resources
Related Products
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Enzymes for cell dissociation
-
Recombinant Trypsin, Liquid Solution, GMP Grade
- Bovine Trypsin, recombinant, expressed in corn, lyophilized powder
Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.