Provis Biolabs Recombinant Trypsin, Lyophilized Powder, GMP Grade, distributed by Ilex Life Sciences LLC

Recombinant Trypsin, Lyophilized Powder, GMP Grade

Regular price
Sale price
Regular price
Unit price
Shipping calculated at checkout.


Recombinant Trypsin, GMP Grade, expressed in Pichia pastoris, is an enzyme with 223 amino acids and molecular weight of 23.5 kDa. Trypsin is a serine protease that cleaves peptide bonds on the carboxyl side of lysine and arginine residues, playing a crucial role in protein digestion and cell culture applications. Recombinant Trypsin offers improved purity, stability, and control compared to traditional animal-derived trypsin.


    • Available as a lyophilized power or liquid solution.
    • Expressed in yeast P. pastoris by recombinant DNA technology.
    • High specific activity ≥180 U/mg.
    • Purity ≥ 70% (β-Trypsin)
    • Optimal pH range from 7.5 to 8.5 for efficient enzymatic activity.
    • Antibiotic-free manufacturing process.
    • Raw materials used are animal component free, TSE/BSE certified, and comply with IP/USP/EP.
    • Manufactured in a GMP certified facility with ISO classified clean rooms, following ICH Q7 principles to meet international standards.


    • Adherent cell dissociation and passaging in cell culture.
    • Protein digestion for mass spectrometry analysis.
    • Recombinant protein production and purification.
    • Tissue engineering and regenerative medicine.
    • Production of insulin.


    Product Category
    Cell Dissociation Enzymes
    Product Type
    CAS Registry No.
    Expressed in Pichia pastoris yeast
    Lyophilized powder
    White to off-white, odorless powder
    Solubility (10 mg/ml)
    Clear colorless solution
    Size Various, bulk sizes on request
    Enzyme Activity by USP Method
    Not less than 2500 USP Units/mg
    Protein Content by UV 280 Method
    95.0% ± 10.0%
    Specific Activity
    Not less than 3800 USP Units/mg (BAEE)
    Purity by RP-HPLC
    1. Not less than 70% for the peak area of β-Trypsin.
    2. Not more than 20% for the peak area of α-Trypsin.
    Yeast Host Cell Protein Residue
    ≤ 0.01%
    Yeast Host Cell DNA Residue
    ≤ 10.0000 ng/mg
    Endotoxin Content (LAL Test)
    ≤ 20.0 EU/mg
    Total Microbial Count (TVAC/TYMC)
    1. TVAC: Not more than 1000 cfu/g
    2. TYMC: Not more than 100 cfu/g
    Pathogens Free of E. coli, Salmonella sp., S. aureus, P. aeruginosa.
    Storage Temperature 2-8°C
    Manufacturer Provis Biolabs pvt Ltd
    Country of Origin India

    Application Note

    Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

    Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.


    Related Products

      Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.