Description
Taq DNA Polymerase Recombinant is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
CAS Registry No.
9012-90-2
Source
Recombinantly produced in E. coli.
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 µm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 µg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 ul.
Formulation
Taq DNA Polymerase solution in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
10x Reaction Buffer with MgCl2
500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15 mM MgCl2. Buffer is optimized for use with 0.2 mM of each dNTP.
10x Reaction Buffer without MgCl2
500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Includes: 10x Reaction Buffer without MgCl2 and separate 25 mM MgCl2 Solution.
Specify your own reaction conditions
Choose either Taq with Mg-free 10x Reaction Buffer and separate 25 mM MgCl2 or Taq with 10x Reaction Buffer containing 15 mM MgCl2.
Storage Buffer
Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol and 1% Triton X-100.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Shipping & Storage
Ships on ice packs. Upon receipt, store the product at the temperature recommended below:
Stable for 5 days at 10°C, for longer period of time store at -20°C.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.