Description
Taq DNA Polymerase Recombinant is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Source
Recombinantly produced in E. coli.
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 µm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 µg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 ul.
Formulation
Taq DNA Polymerase solution in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
10x Reaction Buffer with MgCl2
500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15 mM MgCl2. Buffer is optimized for use with 0.2 mM of each dNTP.
10x Reaction Buffer without MgCl2
500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Includes: 10x Reaction Buffer without MgCl2 and separate 25 mM MgCl2 Solution.
Specify your own reaction conditions
Choose either Taq with Mg-free 10x Reaction Buffer and separate 25 mM MgCl2 or Taq with 10x Reaction Buffer containing 15 mM MgCl2.
Storage Buffer
Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol and 1% Triton X-100.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Shipping & Storage
Ships on ice packs. Upon receipt, store the product at the temperature recommended below:
Stable for 5 days at 10°C, for longer period of time store at -20°C.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.