Description
The ToLuminate PEG Photogel Kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified cross-linker PEG-Link. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on PEG-Link are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination.
Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the norbornene groups on N-Dextran with the same chemical reaction as PEG-Link to provide a cell-adhesive matrix.
3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.
Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels", which may be downloaded below.
Application
- 3D cell cultures
- Bioprinting
Quantity
Up to 4 ml hydrogel.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| N-Dextran |
600 µl | 30 mmol/L | MSDS |
| PEG-Link, lyophilized | 3 x 200 µl* | 20 mmol/L* thiol groups | MSDS |
| 10x CB Buffer (pH 7.2) Phenol Red-free | 600 µl | n/a | |
| LAP, 10 mM | 600 µl | 10 mmol/L | MSDS |
| Water | 2 x 1500 µl | n/a | MSDS |
* Volume/concentration after reconstitution of lyophilizate.
Resources
- Product Datasheet (PDS): ToLuminate PEG Photogel Kit
- Protocol: Preparation of ToLuminate Photogels
- Protocol: Dissolution of Dextran-Based Hydrogels with 3-D Life Dextranase (Recovery of Cells)
Related Products
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.