Description
The 3-D Life Dextranase allows the degradation of dextran-based 3-D Life Hydrogels. It contains dextranase from Chaetomium sp., which cleaves glycosidic bonds of dextran. The brown color of the dextranase solution can vary from lot to lot. This does not interfere with the product's quality.
Important Note: The 3-D Life Dextranase allows the degradation of dextran-based 3-D Life Hydrogels when crosslinked with PEG-Link or CD-Link. It does not allow the
degradation when hydrogels are crosslinked with HyLink.
For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which can all be found at the links below.
Application
Dissolution of dextran-based hydrogels to recover live or fixed cells without proteolytic degradation. 3-D Life Dextranase is used at a 1:20 dilution in the culture supernatant to dissolve dextran-based hydrogels. A detailed description of 3D cell culture application is available in the 3-D Life Hydrogels User Guide.
Quantity
500 µl
Concentration of reactive groups
>4000 units/mL
Resources
- Product Datasheet (PDS): Dextranase
- Material Safety Data Sheet (MSDS): Dextranase
- 3-D Life Hydrogel User Guide
- Protocol: Degradation of dextran-based hydrogels with dextranase
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Other names:
EC 3.2.1.11, 1,6-alpha-D-glucan-6-glucanohydrolase, 6-alpha-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan-6-glucanohydrolase, Alpha-1,6-glucan-6-glucanohydrolase, alpha-D-1,6-glucan-6-glucanohydrolase, alpha-dextranase, alpha-glucanase, AODex, Dex, Dex2, Dex410, Dex49A, DexA, dextran hydrolase, dextranase, dextranase DL 2, dextranase I, dextranase II, dextransucrase, DL 2, endo-dextranase, endodextranase, extracellular dextranase, Fjoh_4430, LSD1, SmDex, Teth39_0264, TPDex
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.