Description
The Active Ghrelin Human/Rat/Mouse ELISA kit, developed and manufactured in Japan by Yanaihara Institute Inc., is used for quantitative measurement of the active form of Ghrelin in plasma samples. This multispecies assay can detect not only octanoylated human Ghrelin but also octanoylated rat/mouse Ghrelin (1-28). This kit is manufactured using the highly specific antibody pairs generated by Dr. Kangawa and by following his protocol. (patent pending: PCT WO 01/07475 A1).
With one assay kit, 40 samples can be measured in duplicate. The 96-well plate in the kit consists of 8-well strips, and the strips can be used separately.
NOTE: This new assay uses the same antibody as the discontinued LSI Medicine Active Ghrelin ELISA (cat. no. 97751).
Summary
Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a peptide consisting of 28-amino acid by Dr. Kenji Kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormone activity.
Other Names
Acylated ghrelin (AG), Acyl-ghrelin, octanoyl ghrelin, octanoylated ghrelin, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UniProtKB# Q9UBU3.
Specifications
| Type | ELISA |
| Sample Type | Plasma (human, rat, or mouse) |
| Sample Volume | 50 μL |
| Assay Range | 2.9 - 184 fmol/mL |
| Assay Run Time | Within 2hr+1hr+0.5hr |
Comparison
The Yanaihara Active Ghrelin ELISA uses the same antibody (manufactured by LSI Medicine) as the discontinued LSI Medicine Active Ghrelin ELISA (Cat. No. 97751), and is thus a suitable alternative product.
Figure 1. Correlation of Active Ghrelin between Yanaihara Institute Active Ghrelin ELISA and discontinued LSI Medicine Active Ghrelin ELISA.
Stability and Storage
Store all of the components at 2-8ºC. The kit is stable under the condition for 12 months from the date of manufacturing. The expiry date is stated on the label of kit.
Usage
This assay is intended for in vitro laboratory research use only; not for use in diagnostic or therapeutic procedures.
Resources
Summary References
- Kojima, M. et al.: Nature, 402: 656, 1999
- Inui, A.: Nat. Rev. Neurosci., 2: 551, 2001
- Date, Y. et al. : Endocrinology, 141 : 4255, 2000
- Gualillo, O. et al. : Endocrinology, 142: 788, 2001
- Matsumoto, M. et al.: Biochem. Biophys. Res. Commun., 287: 142, 2001
- Hosoda, H. et al.: Biochem. Biophys. Res. Commun., 279: 909, 2000
Related Products
Ilex Life Sciences LLC is an authorized distributor of Yanaihara Active Ghrelin Human/Rat/Mouse ELISA.