Description
3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel.
CD-Link can be used with 3-D Life functionalized polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.
CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP3, MMP7 and MMP9. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.
For using this product please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3D cell cultures.
Quantity
Cat. No. L60-1: When used with polymers of the 3-D Life Hydrogel system up to 2 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Cat. No. L60-3: When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Components
Cat. No. L60-1:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-Link, Lyophilized |
200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilizate. |
*Concentration after reconstitution of lyophilizate. |
Cat. No. L60-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-Link, Lyophilized | 3 x 200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilizate. | *Concentration after reconstitution of lyophilizate. |
Resources
- Product Datasheet (PDS): 3-D Life CD-Link (200 µl)
- Product Datasheet (PDS): 3-D Life CD-Link (3x 200 µl)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels
- Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels
- Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Product References
- Guenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” Frontiers in immunology vol. 15 1434030. 15 Aug. 2024, doi:10.3389/fimmu.2024.1434030
- Chen, Jeng-Chang et al. “Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.” Stem cell research & therapy vol. 14,1 290. 5 Oct. 2023, doi:10.1186/s13287-023-03517-y
- Albrecht, Marco et al. “Mechanistically Coupled PK (MCPK) Model to Describe Enzyme Induction and Occupancy Dependent DDI of Dabrafenib Metabolism.” Pharmaceutics vol. 14,2 310. 28 Jan. 2022, doi:10.3390/pharmaceutics14020310
- Del Mistro, Greta et al. “Focal adhesion kinase plays a dual role in TRAIL resistance and metastatic outgrowth of malignant melanoma.” Cell death & disease vol. 13,1 54. 12 Jan. 2022, doi:10.1038/s41419-022-04502-8
- Kuehlbach, Claudia et al. “Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model.” Bioengineering (Basel, Switzerland) vol. 8,11 186. 15 Nov. 2021, doi:10.3390/bioengineering8110186
- Sun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” STAR protocols vol. 2,4 100794. 29 Sep. 2021, doi:10.1016/j.xpro.2021.100794
- Nair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” Biosensors vol. 11,9 314. 3 Sep. 2021, doi:10.3390/bios11090314
- Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390/ma12203391
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.