Description
3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel.
CD-Link can be used with 3-D Life functionalized polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.
CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP3, MMP7 and MMP9. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.
For using this product please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3D cell cultures.
Quantity
Cat. No. L60-1: When used with polymers of the 3-D Life Hydrogel system up to 2 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Cat. No. L60-3: When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Components
Cat. No. L60-1:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-Link, Lyophilized |
200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilizate. |
*Concentration after reconstitution of lyophilizate. |
Cat. No. L60-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-Link, Lyophilized | 3 x 200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilizate. | *Concentration after reconstitution of lyophilizate. |
Resources
- Product Datasheet (PDS): 3-D Life CD-Link (200 µl)
- Product Datasheet (PDS): 3-D Life CD-Link (3x 200 µl)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels
- Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels
- Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.