Introduction
The 3-D Life GFOGER-3 Peptide contains motifs from collagen, promoting adhesion to cellular integrin receptors. 3-D Life GFOGER-3 spontaneously forms a triple helix mimicking the collagen triple helical structure. Its termini are modified with thiol groups for attachment to the hydrogel matrix.
Description
The 3-D Life GFOGER-3 Peptide spontaneously forms stable triple helices like those in fibrillar collagens including collagen type I, II, IV and XI. Its amino acid sequence is derived from the triple helical building block, promoting cell adhesion through integrin receptors, including α1β1 and α2β1, α10β1, and α11β1. At each end the peptide is modified with a SH-group which binds to the SH-reactive group of 3-D Life polymers. Once hydrogels are formed by crosslinking the polymers with 3-D Life crosslinkers, the immobilized peptide promotes adhesion of cells carrying the appropriate integrin receptors. GFOGER-3 mediates cell adhesion within the gel or on top of the gel.
For instructions of hydrogel preparations, please consult General Protocol GP-2 “Preparation of 3-D Life Slow Gelling Hydrogels” and General Protocol GP-4 "Preparation of 3-D Life Hyaluronic Acid Hydrogels", which may be downloaded below. For general information consult the 3-D Life Hydrogels User Guide below.
Application
Modification of 3-D Life Hydrogels with cell-adhesive properties. Culture of cells requiring a cell-adhesive matrix based on the GFOGER motif. Culture of cells in 3-D or on top of hydrogels.
Quantity
Cat. No. P12-1: 2.4 mg
Cat. No. P12-3: 3 x 2.4 mg
Components
Cat. No. P12-1:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| GFOGER-3 Peptide, lyophilized | 120 µl* | 20 mg/ml* | MSDS |
| Water | 600 µl | n/a | MSDS |
| *Volume/concentration after reconstitution of lyophilizate. | |||
Cat. No. P12-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| GFOGER-3 Peptide, lyophilized | 3 x 120 µl* | 20 mg/ml* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilizate. | |||
Resources
- Product Datasheet (PDS): 3-D Life GFOGER-3 Peptide (2.4 mg)
- Product Datasheet (PDS): 3-D Life GFOGER-3 Peptide (3 x 2.4 mg)
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- Protocol: Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.