Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Mouse, E. coli Recombinant Protein

A32220005

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Description

Our bioactive Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Mouse, E. coli Recombinant Protein is a single, non-glycosylated, polypeptide chain containing 125 amino acids and a molecular mass of 14285.35 Dalton. GM-CSF Mouse is purified by proprietary chromatographic techniques.

Summary

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a monomeric glycoprotein that functions as a cytokine. It is a white blood cell growth factor. GM-CSF is a hematopoietic growth and differentiation factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages).

Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Other Names

Murine GM-CSF, Colony-Stimulating Factor, CSF-2, CSF2, MGI-1GM, Pluripoietin-α, molgramostin, sargramostim, Csfgm, Gm-CSf, GMCSF, MGI-IGM

Source

E. coli

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. The mouse GM-CSF was lyophilized with no additives.

Purity

Greater than 98.0% as determined by analysis by RP-HPLC and SDS-PAGE.

Biological Activity

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

Protein Content

GM-CSF quantitation was carried out by two independent methods:

UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.

Reconstitution

It is recommended to reconstitute the lyophilized GM-CSF in sterile 20mM AcOH (acetic Acid) not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized GM-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Usage

This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Resources

Product Datasheet (PDS)

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