Panexin BMM Defined Serum Substitute, w/o: Glucocorticoide

Description

PAN-Biotech Panexin BMM is a fully defined serum replacement for the cultivation of macrophages from mouse bone marrow (murine bone marrow derived macrophages, BMM) under serum-free conditions. The ready-to-use sterile solution in a final concentration of 5% is added to the basal medium RPMI 1640, supplemented with 50 μM Mercaptoethanol and 2 ng/ml GM-CSF murine recombinant.

Composition

Panexin BMM contains purified proteins (<3% w/v), lipids, salts, amino acids, trace elements, attachment factors, hormones and animal-derived components (<12% w/v). It contains no growth factors, undefined hydrolysates or lysates (e. g. Peptones).

Advantages

Panexin BMM has been developed for the generation of macrophages from mouse bone marrow under serum-free conditions. The results will be more comparable, as undefined components (like in serum-containing cultures) are eliminated. In Panexin BMM, matured macrophages will show excellent attachment capabilities. This achieves standardized conditions and reproducible results.

Application Notes

Panexin BMM is added to the basal medium RPMI 1640 in a final concentration of 5% and has to be supplemented with 50 µM Mercaptoethanol and 2 ng/ml GM-CSF murine rec. For example:

• 475 ml RPMI 1640
• 25 ml Panexin BMM
• 1 µg GM-CSF
• 1.75 µl Mercaptoethanol

After the isolation of the bone marrow from a mouse, a single cell suspension will be achieved by frequent pipetting. Centrifuge the cells at 200g for 10 minutes. Resuspend the isolated cells in serum-free medium and seed the cells in three T75 cell culture flasks with 20 ml medium. The incubation is done at 37°C and 5% CO2. Feeding of the cells should be done on day 5 and 7 by an exchange of 10 ml of medium with fresh medium. After 10 days, the cells can be harvested.

Storage & Stability

Store: at -20°C, in the dark.

Stability: 2 years from date of production

Usage

This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Other Names

• Product Datasheet (PDS)
  
• Material Safety Data Sheet (MSDS)
• Product References
• Key Reference: Eske, Kristin et al. “Generation of murine bone marrow derived macrophages in a standardised serum-free cell culture system.” Journal of immunological methods vol. 342,1-2 (2009): 13-9. doi:10.1016/j.jim.2008.11.011

Related Products

• Panexin Serum Replacements
• GM-CSF Mouse, E. coli Recombinant Protein
  
• 2-Mercaptoethanol 50 mM in DPBS
• RPMI 1640

Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech GmbH.