PAN-Biotech Panexin BMM is a fully defined serum replacement for the cultivation of macrophages from mouse bone marrow (murine bone marrow derived macrophages, BMM) under serum-free conditions. The ready-to-use sterile solution in a final concentration of 5% is added to the basal medium RPMI 1640, supplemented with 50 μM Mercaptoethanol and 2 ng/ml GM-CSF murine recombinant.
Panexin BMM contains purified proteins (<3% w/v), lipids, salts, amino acids, trace elements, attachment factors, hormones and animal-derived components (<12% w/v). It contains no growth factors, undefined hydrolysates or lysates (e. g. Peptones).
Panexin BMM has been developed for the generation of macrophages from mouse bone marrow under serum-free conditions. The results will be more comparable, as undefined components (like in serum-containing cultures) are eliminated. In Panexin BMM, matured macrophages will show excellent attachment capabilities. This achieves standardized conditions and reproducible results.
Panexin BMM is added to the basal medium RPMI 1640 in a final concentration of 5% and has to be supplemented with 50 µM Mercaptoethanol and 2 ng/ml GM-CSF murine rec. For example:
- 475 ml RPMI 1640
- 25 ml Panexin BMM
- 1 µg GM-CSF
- 1.75 µl Mercaptoethanol
Storage & Stability
Store: at -20°C, in the dark.
Stability: 2 years from date of production
This product is intended for Laboratory Research Use Only. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
FBS replacement, FBS substitute, serum substitute
Product Datasheet (PDS)
- Material Safety Data Sheet (MSDS)
- Product References
- Key Reference: Eske, Kristin et al. “Generation of murine bone marrow derived macrophages in a standardised serum-free cell culture system.” Journal of immunological methods vol. 342,1-2 (2009): 13-9. doi:10.1016/j.jim.2008.11.011
Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech GmbH.