Matrix Gla Protein (MGP) Human recombinantly produced in E. coli is a single, non-glycosylated polypeptide chain containing 98 amino acids (20-96) and has a molecular mass of 11.8 kDa. The MGP is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Matrix Gla protein (MGP) is a member of a family of vitamin-K2 dependent, Gla-containing proteins. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. MGP is vital for the regulation of calcification in the extracellular matrix, particularly in cartilage and arteries.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF
Greater than 90%
Sterile Filtered colorless solution. The MGP solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Shipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
This product is intended to Laboratory Research Use Only. This product may not be used a drug, agricultural product, food additives.
Matrix Gla protein, Cell growth-inhibiting gene 36 protein, GIG36, MGLAP, NTI.
Source: Nishimoto, S. K., & Nishimoto, M. (2014). Matrix gla protein binds to fibronectin and enhances cell attachment and spreading on fibronectin. International journal of cell biology, 2014, 807013. https://doi.org/10.1155/2014/807013
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