Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel, 100 mg vial, cat. no. hPLMA/100, distributed by Ilex Life Sciences.
Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel, 500 mg vial, cat. no. hPLMA/500, distributed by Ilex Life Sciences.
  • Load image into Gallery viewer, Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel, 100 mg vial, cat. no. hPLMA/100, distributed by Ilex Life Sciences.
  • Load image into Gallery viewer, Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel, 500 mg vial, cat. no. hPLMA/500, distributed by Ilex Life Sciences.

Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel

hPLMA/100
$250.00
$250.00
$296.00
Unavailable
Shipping calculated at checkout.
Metatissue Logo

Introduction

Metatissue® hPLMA Photopolymerizable Hydrogel is a human based, xeno-free, bioactive platelet lysates-derived platform that provides physiologically relevant microenvironments for cell culture. The precursor solution is easily prepared by reconstituting hPLMA lyophilizate in an aqueous solution with a photoinitiator. Afterwards it can be cured upon light exposure to form a hydrogel with tunable mechanical properties. hPLMA Photopolymerizable Hydrogels provide great control over gelation time and matrix stiffness, enabling researchers to create the cell culture environment that better fits their needs.

Applications:

  • 3D cell culture: Cell growth and expansion.
  • Tissue engineering: Platform that supports tissue regeneration.
  • Disease modeling: Drug discovery and screening.
  • Cancer research: Tumor-like microenvironments.
  • Stem cell research: Suitable for stem cell proliferation.
  • Microtissue development: Tissue morphogenesis and homeostatis.

Advantages:

  • Human-derived: Multiple native human-derived proteins and growth factors provide a xeno-free alternative to animal products.
  • Mechanically tuneable: Mechanical properties can be easily adjusted to obtain several tissue stiffness simulations.
  • Photocrosslinkable: The precursor solution can be cured upon light exposure to induce hydrogel crosslinking. Its temperature stability allows for easier handling.
  • Versatile: Easily processed in multiple geometries and in microarrays amenable to organ-on-a-chip systems designed for high throughput screening applications.
  • Reproducible: The materials are prepared from multiple donor units that are pooled to minimize lot-to-lot variation.
  • Personalized: Under request, specific products may be prepared with samples from a specific donor, enriched or depleted in specific target molecules.

hPLMA Material

Metatissue® hPLMA Photopolymerizable Hydrogel is composed of several proteins, including growth factors and cytokines. These bioactive proteins are known to be key components involved in cell adhesion and proliferation processes.

Main Components:

  • Serum albumin
  • Epidermal growth factor (EGF)
  • Platelet-derived growth factor (PDGF)
  • Fibrinogen
  • Vascular endothelial growth factor (VEGF)
  • Complement C3
  • Serotransferrin

Source:

hPLMA is manufactured from platelet units obtained from screened healthy donors designated for therapeutic transfusion. Each donor undergoes testing, and non-reactivity is confirmed for HBsAg, anti-HBc, anti-HIV-1/2, anti-HCV, anti-HTLV-1/2, anti-T. cruzi, HIV-1, HCV, HBV, WNV nucleic acid testing, and syphilis micro hemagglutination assay. It is essential to observe universal precautions for handling and disposing of biological products while working with hPLMA.

Photoinitiated Polymerization:

hPLMA is chemically modified with photoresponsive groups to polymerize only when exposed to ultraviolet (UV) or visible light. Therefore, a photoinitiator is required to initiate the polymerization process (not provided). For recommended photoinitiators, please refer to the Product Datasheet (PDS).

Specifications

Product Category: Hydrogels, scaffolds, biomatrices
Product Type:
 Hydrogel
Liquid/Powder: Lyophilized powder
Size: 100 mg or 500 mg vials
Storage: 2-8°C
Manufacturer: Metatissue®
Country of Origin: Portugal

hPLMA Validation in Cell Culture

Validated Cell Types

These are some of the human cells already validated with the hPLMA product:

  • Adipose stem cells
  • Lung fibroblasts
  • Osteosarcoma cancer cell line
  • Cardiomyocytes
  • Smooth muscle cells
  • Urothelial cells
  • Bone marrow stem cells

Tunable Stiffness

hPLMA Photopolymerizable Hydrogel has tunable mechanical properties adjusted by the concentration of hPLMA lyophilizate in the precursor solution. It is therefore possible to obtain hydrogels with different stiffness to closely mimic the mechanical properties of various native tissues.

hPLMA Photopolymerizable Hydrogel has tunable mechanical properties adjusted by the concentration of hPLMA lyophilizate in the precursor solution. It is therefore possible to obtain hydrogels with different stiffness to closely mimic the mechanical properties of various native tissues.

Cell Viability & Proliferation

Encapsulated stem cells maintain their viability for at least 7 days and are able to proliferate inside the hydrogels.

Figure 1 - Representative fluorescence images for hASCs encapsulated in hPLMA 15% w/v live/dead[1].

Figure 2 - DNA and MTS results for hASCs at 1, 3, and 7 days encapsulated cell culture [1].Cell Invasion & Proliferation

hPLMA hydrogels support the tumor invasion behavior of cancer cell lines (MG-63, SaOS-2, and A549) and hBM-MSC spheroids. Different cell lines present different invasiveness ability, reflected in hPLMA hydrogels and present a better performance than the animal-derived platform counterpart (Matrigel).

Figure 3 - Representative fluorescence images for A549, SaOS-2, MG-63 and hBM-MSC encapsulated in hPLMA 15% w/v and Matrigel labelled with Dapi/phalloidin[2].

Figure 4 - Invasion speed results for A549, SaOS-2, MG-63 and hBM-MSC encapsulated in hPLMA 15% w/v and Matrigel.Dynamic conditions enabled growth and invasiveness ability of hPLMA Hydrogels by metastatic cancer cells.

Figure 5 - Metastatic Cancer Cells Thrive amidst Dynamic Conditions within hPLMA Hydrogels[4].

3D Bioprinting by Extrusion

The 3D bioprinted constructs with hPLMA, Laminaran-methacrylate (LamMA), Glucoamylase, and hASC, cultured in glucose-free medium, exhibited high cell viability and cell elongated morphology, highlighting the potential of hPLMA as a glucose self-supplying bioink.

Figure 6 - Schematic illustration of applying self-sustaining hydrogel precursor as a bioink for 3D printing using agarose support bath. Representative confocal images of live-dead assay of 3D printed ring structure (Rectilinear pattern, 80% infill) after 7 days of culture in DMEM no glucose [5].

Documents

Usage

This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Ilex Life Sciences LLC is an authorized worldwide distributor of Metatissue® products.