Description
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.
Suitability
Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.
Specifications
Cat. No. P10-024100
| Ca/Mg Content: | Without Ca and Mg |
|
Concentration |
10x concentrated |
| Trypsin Concentration | 0.50% Trypsin |
|
EDTA Concentration |
0.20% (5.3 mM) EDTA |
|
Liquid/Powder |
Liquid |
| Phenol Red | Without phenol red |
| Product Category | Enzymes for cell dissociation |
| Product Type | Trypsin-EDTA |
| Size | 100 ml |
| Sterile | Yes |
| Storage Temperature | -20°C |
| In Solution | In PBS |
| Contaminants | Tested negative for mycoplasma and PPV. |
| Manufacturer | PAN-Biotech GmbH |
| Country of Origin | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.
Resources
Product References
- Maiti, Samarpan et al. “Hsf1 and the molecular chaperone Hsp90 support a 'rewiring stress response' leading to an adaptive cell size increase in chronic stress.” eLife vol. 12 RP88658. 7 Dec. 2023, doi:10.7554/eLife.88658
- Feles, Sebastian et al. “Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies.” Methods and protocols vol. 5,4 58. 12 Jul. 2022, doi:10.3390/mps5040058
- Vafeiadou, Vasiliki et al. “Hyperactivation of MAPK Induces Tamoxifen Resistance in SPRED2-Deficient ERα-Positive Breast Cancer.” Cancers vol. 14,4 954. 14 Feb. 2022, doi:10.3390/cancers14040954
- Stahlhut, Maike et al. “Multimodal Lentiviral Vectors for Pharmacologically Controlled Switching Between Constitutive Single Gene Expression and Tetracycline-Regulated Multiple Gene Collaboration.” Human gene therapy methods vol. 28,4 (2017): 191-204. doi:10.1089/hgtb.2017.073
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