Description
PAN-Biotech Trypsin 0.05% / EDTA 4 Na 0.02% in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA•4Na in an HBSS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.
Suitability
Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.
Specifications
Cat. No. P10-040100
| Ca/Mg Content: | Without Ca and Mg |
|
Concentration |
1x |
| Trypsin Concentration | 0.05% Trypsin |
|
EDTA Concentration |
0.02% EDTA-Na4 |
|
Liquid/Powder |
Liquid |
| Phenol Red | With phenol red |
| Product Category | Enzymes for cell dissociation |
| Product Type | Trypsin-EDTA |
| Size | Various |
| Sterile | Yes |
| Storage Temperature | -20°C |
| In Solution | In HBSS (Hanks' Balanced Salt Solution) |
| Contaminants | Tested negative for mycoplasma and PPV. |
| Manufacturer | PAN-Biotech GmbH |
| Country of Origin | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.
Resources
Product References
- Han, Zhe et al. “A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells.” Bio-protocol vol. 12,16 e4490. 20 Aug. 2022, doi:10.21769/BioProtoc.4490
- Di Genova, Cecilia et al. “Production, Titration, Neutralisation, Storage and Lyophilisation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Lentiviral Pseudotypes.” Bio-protocol vol. 11,21 e4236. 5 Nov. 2021, doi:10.21769/BioProtoc.4236
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