Description
ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.
When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.
When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.
For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels", which may be downloaded below.
Application
- 3D cell cultures
- Bioprinting
Quantity
Without peptide modification:
- Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
- Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced if peptide modifications are added.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| N-Dextran |
2 x 600 µl | 30 mmol/L | MSDS |
| 10x CB Buffer (pH 7.2) Phenol Red-free | 2 x 600 µl | n/a | |
| LAP, 10 mM | 2 x 600 µl | 10 mmol/L | MSDS |
| Water | 3 x 1500 µl | n/a | MSDS |
Resources
- Product Datasheet (PDS): ToLuminate N-Dextran
- Protocol: Preparation of ToLuminate Photogels
- Protocol: Dissolution of Dextran-Based Hydrogels with 3-D Life Dextranase (Recovery of Cells)
Related Products
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.