Yamasa SP-D Kit EIA II 96 well microplate
Yamasa SP-D Kit EIA II example of standard curve
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Yamasa SP-D Kit EIA II (96 wells) - Human Surfactant Protein D ELISA

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Description

The SP-D Kit EIA II, developed and manufactured in Japan by Yamasa Corporation, is a quantitative immunoassay for determination of human surfactant protein D (SP-D) in serum.

SP-D is a 43-kDa protein with a collagen-like domain. Its four trimers assemble in vivo to form a dodecamer. SP-D is known as a lung-specific protein because it is mainly produced by alveolar type II epithelial and club cells.
SP-D plays important roles in the biological defense mechanism in the airway-alveolar system. For example, it promotes bacterial phagocytosis by alveolar macrophages. On the other hand, SP-D in the alveoli is transferred to the blood and increased in several types of interstitial pneumonia, such as idiopathic pulmonary fibrosis, collagen disease lung, and drug-induced lung injury.

Type

Assay principle of this kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA).

Other names

Pulmonary surfactant-associated protein D, SFTPD, PSP-D, Collectin-7, Lung surfactant protein D, SFTP4

Principle of Method

This kit is intended for measuring serum SP-D by enzyme immunoassay (EIA) using the microplate solid-phase method. A sample is added to a microplate with an immobilized mouse anti-human SP-D monoclonal antibody. Then, it is reacted with a horseradish peroxidase-labeled mouse anti-human SP-D monoclonal antibody with a recognition site different from that of the solid-phase antibody. In this process, a solid-phase antibody-antigen (SP-D)-labeled antibody complex is formed. Subsequently, the amount of SP-D in the sample is quantified from absorbance at 450 nm in the enzyme-substrate reaction (color reaction).

Outline of Yamasa SP-D Kit EIA Protocol

Sample Types

Serum

Sample Volume

Serum: 9.09 µL (actual amount of sample loaded in well after dilution)

Standard Curve


Example of Yamasa SP-D Kit EIA II standard curve

Calibration Range:  1.56-100 ng/mL

Resources

Want to learn more about Irisin? We have compiled the links below which contain information that you may find interesting:

References to Summary

  • Persson A et al. (1988). Biochemistry. 27. 8576.
  • Nagae H et al. (1996). Jpn J Med Pharm Sci. 36. 803-808.
  • Honda Y et al. (1996). Jpn J Med Pharm Sci. 36. 809-815.
  • Tanaka M et al. (2008). Jpn J Med Pharm Sci. 59. 439-446.

 Ilex Life Sciences LLC is an authorized distributor of Yamasa SP-D Kit EIA II.