96-well microplate
Rat/Mouse SP-D Kit EIA II Standard Curve
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Yamasa Rat/Mouse SP-D Kit EIA II (96 wells) - Surfactant Protein D ELISA

80103
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Description

The Rat/Mouse SP-D Kit EIA II, developed and manufactured in Japan by Yamasa Corporation, is a quantitative immunoassay for determination of rat or mouse surfactant protein D (SP-D) in rat or mouse serum or bronchoalveolar lavage fluids (BALF). This ELISA allows highly sensitive measurement of rat serum SP-D. In addition, the antibody used in the ELISA also reacts with mouse SP-D, allowing the measurement of mouse SP-D.

SP-D is a 43-kDa protein with a collagen-like domain. Its four trimers assemble in vivo to form a dodecamer. SP-D is known as a lung-specific protein because it is mainly produced by alveolar type II epithelial and club cells.
SP-D plays important roles in the biological defense mechanism in the airway-alveolar system. For example, it promotes bacterial phagocytosis by alveolar macrophages. On the other hand, SP-D in the alveoli is transferred to the blood and increased in several types of interstitial pneumonia, such as idiopathic pulmonary fibrosis, collagen disease lung, and drug-induced lung injury.

To elucidate the functions of SP-D in vivo, animal experiments are indispensable. This product that allows highly sensitive measurement of rat/mouse SP-D is useful to investigate SP-D. In addition, a study using rats and mice with bleomycin-induced lung fibrosis demonstrated that SP-D may serve as a serum marker to monitor lung injury, suggesting that our product may be useful for detecting lung injuries in various animal experiments.

Type

Sandwich ELISA

Other names

Pulmonary surfactant-associated protein D, SFTPD, PSP-D, Collectin-7, Lung surfactant protein D, SFTP4

Principle of Method

Assay principle of this kit is the solid phase enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies against rat SP-D and rat SP-D as a standard material. Both anti rat SP-D antibodies also can react to mouse SP-D. It is possible to measure the concentration of rat and mouse SP-D in serum and/or bronchoalveolar lavage fluid (BALF) by this kit.

Sample Types

Serum, bronchoalveolar lavage fluids (BALF)

Sample Volume

Rat Serum: 100 µL (after 50-fold dilution)

Mouse Serum: 100 µL (after 10-fold dilution)

Rat or Mouse BALF: 100 µL (after 100-fold dilution)

Standard Curve


Example of standard curve with the Yamasa Rat/Mouse SP-D EIA II Kit
  • Rat SP-D levels can be measured in a range of 0.47-30 ng/mL.

Storage

Store all components at 2-8 °C.

Resources

Want to learn more about SP-D? We have compiled the links below which contain information that you may find interesting:

References to Summary

  • Persson A et al. (1988). Biochemistry. 27. 8576.
  • Nagae H et al. (1996). Jpn J Med Pharm Sci. 36. 803-808.
  • Honda Y et al. (1996). Jpn J Med Pharm Sci. 36. 809-815.
  • Tanaka M et al. (2008). Jpn J Med Pharm Sci. 59. 439-446.

 Ilex Life Sciences LLC is an authorized distributor of Yamasa SP-D Kit EIA II.