Description
The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible 3-D Life Hydrogels.
Its major components are the polymeric SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.
For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3D cell cultures
Quantity
Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| SG-PVA |
170 µl | 30 mmol/L | MSDS |
| PEG-Link, lyophilized | 200 µl* | 20 mmol/L* | MSDS |
| 10x CB Buffer (pH 7.2) | 200 µl | n.a. | MSDS |
| Water | 2 x 1500 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilizate. | |||
Resources
- Product Datasheet (PDS): 3-D Life PVA-PEG Hydrogel SG Kit
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Product References
- Narasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” bioRxiv : the preprint server for biology 2024.07.28.605514. 29 Jul. 2024, doi:10.1101/2024.07.28.605514. Preprint.
- Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389/fbioe.2019.00055
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.