3-D Life 10x CB Buffer (pH 7.2), hydrogel accesory, cat. no. B20-3, manufactured by Cellendes GmbH and distributed by Ilex Life Sciences.
3-D Life 10x CB Buffer (pH 7.2)
  • Load image into Gallery viewer, 3-D Life 10x CB Buffer (pH 7.2), hydrogel accesory, cat. no. B20-3, manufactured by Cellendes GmbH and distributed by Ilex Life Sciences.
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3-D Life 10x CB Buffer (pH 7.2)

B20-3
$34.00
$34.00
$39.00
Unavailable
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Description

3-D Life 10x CB-Buffer (pH 7.2) is a component of the 3-D Life Hydrogel system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system. It is used for the formation of slow gelling 3-D Life Hydrogels where it provides a cell compatible environment at pH 7.2. In conjunction with 3-D Life 10x CB-Buffer (pH 5.5) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.

For instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.

Application

Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures.

Quantity

600 µl.

Sufficient for at least 6 ml 3-D Life Hydrogel.

Resources

Product References

  • Sun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” STAR protocols vol. 2,4 100794. 29 Sep. 2021, doi:10.1016/j.xpro.2021.100794
  • Nair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” Biosensors vol. 11,9 314. 3 Sep. 2021, doi:10.3390/bios11090314
  • Georgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” BMC developmental biology vol. 20,1 4. 26 Feb. 2020, doi:10.1186/s12861-020-0209-5
  • Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389/fbioe.2019.00055

Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.