Description
The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of 3-D Life hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells.
The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the gel if they express the indicated MMPs.
3-D Life ToGro hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase Cat. No. D10-1), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. For preparation of gels follow the instructions of the 3-D Life ToGro Protocol GP-3 “Preparation of 3-D Life ToGro Hydrogel”. The document can be downloaded below.
Application
3D cell culture: 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.
Quantity
Allows formation of 2.4 ml 3-D Life ToGro Hydrogel.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| RGD-Dextran, lyophilized | 2 x 870 µl* | n.a. | MSDS |
| CD-Link, lyophilized | 200 µl* | 20 mmol/L* | MSDS |
| Reconstitution Buffer | 2 x 900 µl | n.a. | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilisate. | |||
Resources
- Product Datasheet (PDS): 3-D Life ToGro
- Protocol: 3-D Cell Culture with 3-D Life ToGro Hydrogel
- Protocol: Degradation of dextran-based hydrogels with dextranase
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Product References
- Chen, Jeng-Chang et al. “Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.” Stem cell research & therapy vol. 14,1 290. 5 Oct. 2023, doi:10.1186/s13287-023-03517-y
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