PAN-Biotech Panserin 401, Serum-free Allround Medium, w: L-Glutamine, 500 ml bottle, cell culture media, cat. no. P04-710401, manufactured by PAN-Biotech GmbH and distributed by Ilex Life Sciences LLC.

Panserin 401, Serum-free Allround Medium, w: L-Glutamine

P04-710401
Regular price
$83.00
Sale price
$83.00
Regular price
$98.00
Unavailable
Unit price
per 
Shipping calculated at checkout.

Introduction

PAN-Biotech Panserin 401 is a fully defined ready-to-use medium for the serum-free cultivation of a multitude of adherent and non-adherent cells.

Composition

Based on Iscove's MEM (IMEM), trace elements, albumin, cholesterol, soy lipids and vitamins were added to the medium. It does not contain any growth or attachment factors. Protein and animal-derived components < 0.2% w/v. Hydrolysates < 0.001% w/v.

Application

Panserin 401 is a multi-purpose medium suitable for a variety of cells. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 401 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities.

Among others, the following cells have been cultivated successfully in Panserin 401:

  • Hybridoma
  • Lymphocytes
  • Macrophages
  • Fibroblasts
  • Melanocytes
  • Carcinoma cells
  • HEK-cells
  • HeLa-cells
  • CHO-cells
  • Megakaryocytic cell lines (HEL, Dami, CMK)
  • HL60-cells
Cell Type: Allround Adherent / Suspension Cells
Culture Type: Adherent & Suspension Culture
Liquid / Powder: liquid
Product Category: Serum-Free Media
Size: 500 ml/bottle
Sterile: Yes
Storage Temprature: +2°C - +8°C
Manufacturer: PAN-Biotech GmbH
Country of Origin: Germany

 

PAN-Biotech Panserin 401 can be used for suspension or adherent cell culture.

Documents

Product References

  • Gomez-Zepeda, David et al. “Thunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model.” Nature communications vol. 15,1 2288. 13 Mar. 2024, doi:10.1038/s41467-024-46380-y
  • Nassauer, Larissa et al. “Protection from cisplatin-induced hearing loss with lentiviral vector-mediated ectopic expression of the anti-apoptotic protein BCL-XL.” Molecular therapy. Nucleic acids vol. 35,1 102157. 19 Feb. 2024, doi:10.1016/j.omtn.2024.102157
  • Volkmar, Michael et al. “Identification of TRDV-TRAJ V domains in human and mouse T-cell receptor repertoires.” Frontiers in immunology vol. 14 1286688. 23 Nov. 2023, doi:10.3389/fimmu.2023.1286688
  • Schwieger, Jana et al. “"Of mice and men": the relevance of Cometin and Erythropoietin origin for its effects on murine spiral ganglion neuron survival and neurite outgrowth in vitro.” Frontiers in neuroscience vol. 17 1224463. 10 Aug. 2023, doi:10.3389/fnins.2023.1224463
  • Berger, Elisabeth et al. “In vitro impact of platinum nanoparticles on inner ear related cell culture models.” PloS one vol. 18,4 e0284794. 24 Apr. 2023, doi:10.1371/journal.pone.0284794
  • Safadi, Daed El et al. “Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection.” Viruses vol. 15,2 364. 27 Jan. 2023, doi:10.3390/v15020364
  • Wieland, Lisa et al. “Epstein-Barr Virus-Induced Genes and Endogenous Retroviruses in Immortalized B Cells from Patients with Multiple Sclerosis.” Cells vol. 11,22 3619. 15 Nov. 2022, doi:10.3390/cells11223619
  • Ogishi, Masato et al. “Impaired IL-23-dependent induction of IFN-γ underlies mycobacterial disease in patients with inherited TYK2 deficiency.” The Journal of experimental medicine vol. 219,10 (2022): e20220094. doi:10.1084/jem.20220094
  • Schnell, Annika P et al. “HDAC inhibitors Panobinostat and Romidepsin enhance tax transcription in HTLV-1-infected cell lines and freshly isolated patients' T-cells.” Frontiers in immunology vol. 13 978800. 16 Aug. 2022, doi:10.3389/fimmu.2022.978800
  • Schwieger, Jana et al. “3D Printed Cell Culture Chamber for Testing the Effect of Pump-Based Chronic Drug Delivery on Inner Ear Tissue.” Biomolecules vol. 12,4 589. 17 Apr. 2022, doi:10.3390/biom12040589
  • Wulf, Katharina et al. “PLLA Coating of Active Implants for Dual Drug Release.” Molecules (Basel, Switzerland) vol. 27,4 1417. 19 Feb. 2022, doi:10.3390/molecules27041417
  • Wille, Inga et al. “Development of Neuronal Guidance Fibers for Stimulating Electrodes: Basic Construction and Delivery of a Growth Factor.” Frontiers in bioengineering and biotechnology vol. 10 776890. 24 Jan. 2022, doi:10.3389/fbioe.2022.776890
  • Heym, Stefanie et al. “Alternative NF-κB Signaling Discriminates Induction of the Tumor Marker Fascin by the Viral Oncoproteins Tax-1 and Tax-2 of Human T-Cell Leukemia Viruses.” Cancers vol. 14,3 537. 21 Jan. 2022, doi:10.3390/cancers14030537
  • Oemer, Gregor et al. “Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells.” Journal of lipid research vol. 62 (2021): 100111. doi:10.1016/j.jlr.2021.100111
  • Schmitt, Heike A et al. “Personalized Proteomics for Precision Diagnostics in Hearing Loss: Disease-Specific Analysis of Human Perilymph by Mass Spectrometry.” ACS omega vol. 6,33 21241-21254. 13 Aug. 2021, doi:10.1021/acsomega.1c01136
  • Lanfermann, Christian et al. “Chlamydia trachomatis Cross-Serovar Protection during Experimental Lung Reinfection in Mice.” Vaccines vol. 9,8 871. 6 Aug. 2021, doi:10.3390/vaccines9080871
  • Ogishi, Masato et al. “Inherited PD-1 deficiency underlies tuberculosis and autoimmunity in a child.” Nature medicine vol. 27,9 (2021): 1646-1654. doi:10.1038/s41591-021-01388-5
  • Frumence, Etienne et al. “Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies.” Journal of immunological methods vol. 495 (2021): 113082. doi:10.1016/j.jim.2021.113082
  • Wieland, Lisa et al. “Overexpression of Endogenous Retroviruses and Malignancy Markers in Neuroblastoma Cell Lines by Medium-Induced Microenvironmental Changes.” Frontiers in oncology vol. 11 637522. 7 May. 2021, doi:10.3389/fonc.2021.637522
  • Deinhardt-Emmer, Stefanie et al. “SARS-CoV-2 causes severe epithelial inflammation and barrier dysfunction.” Journal of virology, vol. 95,10 e00110-21. 26 Feb. 2021, doi:10.1128/JVI.00110-21
  • Harre, Jennifer et al. “Potentiation of Brain-Derived Neurotrophic Factor-Induced Protection of Spiral Ganglion Neurons by C3 Exoenzyme/Rho Inhibitor.” Frontiers in cellular neuroscience vol. 15 602897. 11 Mar. 2021, doi:10.3389/fncel.2021.602897
  • Stein, Stephanie et al. “IL-17A/F enable cholangiocytes to restrict T cell-driven experimental cholangitis by upregulating PD-L1 expression.” Journal of hepatology vol. 74,4 (2021): 919-930. doi:10.1016/j.jhep.2020.10.035
  • Warnecke, Athanasia et al. “Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo.” Clinical and translational medicine vol. 10,8 (2020): e262. doi:10.1002/ctm2.262
  • Hadjadj, Jérôme et al. “Early-onset autoimmunity associated with SOCS1 haploinsufficiency.” Nature communications vol. 11,1 5341. 21 Oct. 2020, doi:10.1038/s41467-020-18925-4
  • Donhauser, Norbert et al. “Transfer of HTLV-1 p8 and Gag to target T-cells depends on VASP, a novel interaction partner of p8.” PLoS pathogens vol. 16,9 e1008879. 30 Sep. 2020, doi:10.1371/journal.ppat.1008879
  • Millen, Sebastian et al. “A novel positive feedback-loop between the HTLV-1 oncoprotein Tax and NF-κB activity in T-cells.” Retrovirology vol. 17,1 30. 10 Sep. 2020, doi:10.1186/s12977-020-00538-w
  • Fournier, Benjamin et al. “Rapid identification and characterization of infected cells in blood during chronic active Epstein-Barr virus infection.” The Journal of experimental medicine vol. 217,11 (2020): e20192262. doi:10.1084/jem.20192262
  • Krüger, Renate et al. “CD70 Deficiency Associated With Chronic Epstein-Barr Virus Infection, Recurrent Airway Infections and Severe Gingivitis in a 24-Year-Old Woman.” Frontiers in immunology vol. 11 1593. 4 Aug. 2020, doi:10.3389/fimmu.2020.01593
  • Wappler, Jessica et al. “Eliminating the need of serum testing using low serum culture conditions for human bone marrow-derived mesenchymal stromal cell expansion.” Biomedical engineering online vol. 12 15. 20 Feb. 2013, doi:10.1186/1475-925X-12-15
  • Montzka, Katrin et al. “Expansion of human bone marrow-derived mesenchymal stromal cells: serum-reduced medium is better than conventional medium.” Cytotherapy vol. 12,5 (2010): 587-92. doi:10.3109/14653240903505814
  • Toptan, T et al. “Rhadinovirus vector-derived human telomerase reverse transcriptase expression in primary T cells.” Gene therapy vol. 17,5 (2010): 653-61. doi:10.1038/gt.2010.3
  • Martín, F et al. “Lentiviral vectors transcriptionally targeted to hematopoietic cells by WASP gene proximal promoter sequences.” Gene therapy vol. 12,8 (2005): 715-23. doi:10.1038/sj.gt.3302457
  • Torres, Pilar S et al. “Contribution of CD3 gamma to TCR regulation and signaling in human mature T lymphocytes.” International immunology vol. 14,11 (2002): 1357-67. doi:10.1093/intimm/dxf095

    Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.