3-D Life Dextran-CD Hydrogel FG Kit

3-D Life Dextran-CD Hydrogel FG Kit

FG91-1
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$272.00
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$272.00
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Description

The 3-D Life Dextran-CD Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.

Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.

Application

3-D cell cultures

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material Quantity Concentration of reactive groups
Mal-Dextran, lyophilized
170 µl 30 mmol/L MSDS
CD-Link, lyophilized 200 µl* 20 mmol/L* MSDS
10x CB Buffer (pH 5.5) 200 µl n.a. MSDS
Water 2 x 1500 µl n.a. MSDS
*Volume/concentration after reconstitution of lyophilisate.

 Resources

Application notes

Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.