3-D Life Dextran-PEG Hydrogel FG Kit

3-D Life Dextran-PEG Hydrogel FG Kit

FG90-1
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$125.00
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$125.00
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Description

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.

Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material Quantity Concentration of reactive groups
Mal-Dextran, lyophilized
170 µl 30 mmol/L MSDS
PEG-Link, lyophilized 200 µl* 20 mmol/L* MSDS
10x CB Buffer (pH 5.5) 200 µl n.a. MSDS
Water 2 x 1500 µl n.a. MSDS
*Volume/concentration after reconstitution of lyophilisate.

 Resources

Application notes

Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.