Description
The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible 3-D Life Hydrogels.
Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.
Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity
Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| Mal-Dextran, lyophilized |
170 µl | 30 mmol/L | MSDS |
| PEG-Link, lyophilized | 200 µl* | 20 mmol/L* | MSDS |
| 10x CB Buffer (pH 5.5) | 200 µl | n.a. | MSDS |
| Water | 2 x 1500 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilizate. | |||
Resources
- Product Datasheet (PDS): 3-D Life Dextran-PEG Hydrogel FG Kit
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Degradation of dextran-based hydrogels with dextranase
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application notes
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Product References
- Guenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” Frontiers in immunology vol. 15 1434030. 15 Aug. 2024, doi:10.3389/fimmu.2024.1434030
- Mohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” Iranian biomedical journal vol. 21,4 (2017): 218-27. doi:10.18869/acadpub.ibj.21.4.218
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.