Description
Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein is a disulfide linked homodimer, glycosylated, polypeptide chain containing 2 x 149 amino acids and has a total molecular mass of 42 kDa. The M-CSF is purified by proprietary chromatographic techniques.
Summary
Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - A0A024R0A1 (CSF1_HUMAN)].
Other Names
Bioactive Human M-CSF, CSF-1, CSF1 gene product, MCSF
Amino Acid Sequence
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ.
Source
Baculovirus infected Silkworm.
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The lyophilized protein (1 mg/ml) was lyophilized with 20mM phosphate buffer, 1% HSA and 3% mannitol.
Purity
Greater than 95.0% as determined by analysis by RP-HPLC and SDS-PAGE.
Biological Activity
The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 3 ng/ml, corresponding to a specific activity of less than 333,333.33 units/mg.
Reconstitution
It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
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