Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein is a non-glycosylated homodimer, containing 2 x 155 amino acids and has a total molecular mass of 36.2 kDa. M-CSF is purified by proprietary chromatographic techniques.
Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - Q8JZQ0 (CSF1_RAT)].
Bioactive Rat M-CSF, CSF-1, CSF1 gene product, MCSF
Amino Acid Sequence
MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP.
Sterile filtered white lyophilized (freeze-dried) powder. Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5.
Greater than 95.0% as determined by SDS-PAGE.
The activity as determined by dose-dependent induction of M-NFS-60 cell proliferation is 1.65 ng/ml. This corresponds to an expected specific activity of 6.1x105 units/mg.
It is recommended to reconstitute the lyophilized M-CSF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
This product is intended to Laboratory Research Use Only. This product may not be used a drug, agricultural product, food additives.
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