Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and has a total molecular mass of 36.4 KD. M-CSF is purified by proprietary chromatographic techniques.
Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - P07141 (CSF1_MOUSE)].
Bioactive Murine M-CSF, CSF-1, CSF1 gene product, MCSF
Amino Acid Sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a sterile (0.2 µm) filtered solution containing 10mM sodium phosphate, 50mM sodium chloride, pH 7.5.
Greater than 95.0% as determined by SDS-PAGE.
The ED50, as calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells is 1.33 ng/ml corresponding to a specific activity of 7.5x105 units/mg.
It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
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